Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1948602..1949215 Replicon chromosome
Accession NZ_CP016972
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag BB988_RS09410 Protein ID WP_003408465.1
Coordinates 1948602..1948991 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag BB988_RS09415 Protein ID WP_003408469.1
Coordinates 1948988..1949215 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB988_RS09375 1944231..1945145 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
BB988_RS09380 1945148..1945978 + 831 WP_003916848.1 cyclase family protein -
BB988_RS09385 1945978..1946328 + 351 WP_003898986.1 cupin domain-containing protein -
BB988_RS09390 1946381..1947199 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
BB988_RS09395 1947213..1947992 + 780 WP_003408460.1 IclR family transcriptional regulator -
BB988_RS09410 1948602..1948991 - 390 WP_003408465.1 PIN domain-containing protein Toxin
BB988_RS09415 1948988..1949215 - 228 WP_003408469.1 antitoxin Antitoxin
BB988_RS09420 1949433..1950917 + 1485 WP_003408473.1 carboxylase -
BB988_RS09425 1950914..1952161 + 1248 WP_003408476.1 serine hydrolase -
BB988_RS09430 1952204..1952623 - 420 WP_003408483.1 hypothetical protein -
BB988_RS09435 1952613..1953323 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T65738 WP_003408465.1 NZ_CP016972:c1948991-1948602 [Mycobacterium tuberculosis H37Ra]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T65738 NZ_CP016972:c1948991-1948602 [Mycobacterium tuberculosis H37Ra]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT65738 WP_003408469.1 NZ_CP016972:c1949215-1948988 [Mycobacterium tuberculosis H37Ra]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT65738 NZ_CP016972:c1949215-1948988 [Mycobacterium tuberculosis H37Ra]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References