Detailed information of TA system TA065545

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 801830..802414
• Replicon: chromosome
• Accession: NZ_CP016937
• Organism: Yersinia enterocolitica strain YE6

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7U7ISC5
• Locus tag: BED33_RS03595
• Protein ID: WP_005165296.1
• Coordinates: 801830..802198 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BED33_RS23030
• Protein ID: WP_129019424.1
• Coordinates: 802262..802414 (-)
• Length: 51 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BED33_RS03575	797138..797944	+	807	Protein_678	ROK family protein	-
BED33_RS03580	798029..798991	+	963	WP_005165289.1	LacI family transcriptional regulator	-
BED33_RS03585	799025..799498	-	474	WP_049528679.1	GNAT family N-acetyltransferase	-
BED33_RS03590	800747..801601	-	855	WP_049528676.1	DUF4942 domain-containing protein	-
BED33_RS03595	801830..802198	-	369	WP_005165296.1	TA system toxin CbtA family protein	Toxin
BED33_RS23030	802262..802414	-	153	WP_129019424.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BED33_RS03605	802483..803686	+	1204	Protein_684	IS256 family transposase	-
BED33_RS03610	803861..805324	+	1464	WP_005166182.1	alpha/beta hydrolase	-
BED33_RS03615	805557..806963	-	1407	WP_069008916.1	filamentous hemagglutinin N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	796055..797086	1031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (1-45)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.55 Da
• Isoelectric Point: 6.4731

>T65545 WP_005165296.1 NZ_CP016937:c802198-801830 [Yersinia enterocolitica]
MQTLPVTSKRAAYACLSPVIVWQSMLTYLLEQHYGLVLSDTEFSDDAVIHEHIDAGISLADALNFTVEKFDLARTDHRGF
SCQEQSPFITSIDILRARRATGLMTRMGYQTITSVIRGGKQA

Nucleotide

• Length: 369 bp

>T65545 NZ_CP016937:c802198-801830 [Yersinia enterocolitica]
ATGCAGACCTTACCTGTCACCTCGAAACGGGCGGCCTACGCTTGCCTATCACCCGTTATTGTCTGGCAATCCATGCTCAC
GTATCTGCTAGAGCAGCATTACGGCCTGGTGCTTAGCGACACCGAGTTCAGTGATGACGCCGTTATCCACGAACATATTG
ATGCCGGTATCTCTCTGGCTGATGCGCTGAATTTCACGGTGGAGAAGTTTGACTTGGCTCGCACCGATCACCGAGGTTTC
TCCTGCCAGGAACAATCTCCCTTTATCACGTCTATCGATATTCTCCGCGCTCGTCGAGCCACCGGTCTGATGACCCGTAT
GGGTTACCAGACCATCACCTCAGTTATCCGGGGAGGAAAGCAGGCATGA

Antitoxin

Protein

• Length: 51 a.a.
• Molecular weight: 5593.44 Da
• Isoelectric Point: 4.5803

>AT65545 WP_129019424.1 NZ_CP016937:c802414-802262 [Yersinia enterocolitica]
MELMLLSGELNPRYQHCVTLYRNGLICEADSLGSQGYVYLAIYPTPQSIA

Nucleotide

• Length: 153 bp

>AT65545 NZ_CP016937:c802414-802262 [Yersinia enterocolitica]
CTGGAACTGATGCTGCTTTCTGGTGAGCTCAATCCCCGTTATCAGCACTGCGTCACGTTGTATCGCAATGGCTTGATCTG
CGAAGCCGATAGCCTGGGATCACAGGGTTATGTGTATCTCGCCATTTATCCTACTCCCCAATCAATTGCATAA

Structures

Toxin

• AlphaFold DB: A0A7U7ISC5