Detailed information of TA system TA065540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 5097..5631
• Replicon: plasmid unnamed2
• Accession: NZ_CP016936
• Organism: Yersinia enterocolitica strain YE7

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0H3NX04
• Locus tag: BED34_RS22035
• Protein ID: WP_014609469.1
• Coordinates: 5097..5396 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P0C2N3
• Locus tag: BED34_RS22040
• Protein ID: WP_005176524.1
• Coordinates: 5389..5631 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BED34_RS22010	1679..2104	-	426	WP_010891235.1	type III secretion system chaperone SycH	-
BED34_RS23920	2308..3479	-	1172	Protein_2	IS3 family transposase	-
BED34_RS22025	3714..4106	-	393	WP_010891236.1	type III secretion system chaperone SycE/YerA	-
BED34_RS22030	4298..4957	+	660	WP_072089396.1	type III secretion system effector GTPase activator YopE	-
BED34_RS22035	5097..5396	-	300	WP_014609469.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BED34_RS22040	5389..5631	-	243	WP_005176524.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BED34_RS23925	5797..6089	-	293	Protein_7	IS6 family transposase	-
BED34_RS22045	6469..7434	-	966	WP_050294053.1	ParB/RepB/Spo0J family plasmid partition protein	-
BED34_RS22050	7431..8597	-	1167	WP_010891239.1	plasmid-partitioning protein SopA	-
BED34_RS22060	9039..9509	+	471	WP_010891240.1	hypothetical protein	-
BED34_RS22065	9570..9788	-	219	WP_013749479.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopH / yopH / sycH / sycE/yerA / yopE / yadA / yopO/ypkA / yopJ/yopP / yopK/yopQ / yopT / sycT / yopM / yopD / yopB / sycD/lcrH / lcrV / lcrG / lcrR / yscV/lcrD / yscY / yscX / sycN / tyeA / lcrE/yopN / yscN / yscO / yscQ / yscR / yscS / yscT / yscU / virG/yscW / yscB / yscC / yscD / yscE / yscF / yscG / yopR/yscH/lcrP / lcrO/yscI / ylpB/yscJ / yscK / yscL / lcrQ/yscM / yopH / sycH / sycE/yerA / yopE / yadA / yadA	1..88902	88902
	-	flank	IS/Tn	-	-	2430..2933	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11664.24 Da
• Isoelectric Point: 5.1053

>T65540 WP_014609469.1 NZ_CP016936:c5396-5097 [Yersinia enterocolitica]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNLELGAECNWICSDMRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDAQDFPEYE

Nucleotide

• Length: 300 bp

>T65540 NZ_CP016936:c5396-5097 [Yersinia enterocolitica]
GTGTATAAACTATCTGAACTGGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCTAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCAGACATGCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGCTCAGGATTTTCCAGAGTATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8856.15 Da
• Isoelectric Point: 6.7232

>AT65540 WP_005176524.1 NZ_CP016936:c5631-5389 [Yersinia enterocolitica]
MARVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNAPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT65540 NZ_CP016936:c5631-5389 [Yersinia enterocolitica]
ATGGCTCGCGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TACTTGCAGGAACAAATGCACCTGTGAGCCATCGTTTAATGGATGAAATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3NX04

Antitoxin

• AlphaFold DB: A1JUB1