65446

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 103808..104330 Replicon plasmid pIncHI1B_DHQP1300920
Accession NZ_CP016921
Organism Klebsiella pneumoniae isolate 11

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag A8C11_RS00980 Protein ID WP_038991638.1
Coordinates 104046..104330 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag A8C11_RS00975 Protein ID WP_004181777.1
Coordinates 103808..104056 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8C11_RS00955 99017..99838 - 822 WP_004181772.1 hypothetical protein -
A8C11_RS00960 99900..100253 - 354 WP_004181774.1 hypothetical protein -
A8C11_RS00965 100398..101384 - 987 WP_040120328.1 hypothetical protein -
A8C11_RS00970 101718..103517 - 1800 WP_043907032.1 ATP-dependent helicase -
A8C11_RS00975 103808..104056 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A8C11_RS00980 104046..104330 + 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A8C11_RS30730 104347..104448 - 102 Protein_108 IS200/IS605 family transposase -
A8C11_RS00985 104484..105710 + 1227 WP_040120327.1 transposase -
A8C11_RS28725 105982..106206 - 225 Protein_110 transposase -
A8C11_RS28730 106285..106713 - 429 WP_077256229.1 IS200/IS605 family transposase -
A8C11_RS00990 106749..107936 + 1188 WP_043907031.1 transposase -
A8C11_RS30735 108035..109130 - 1096 Protein_113 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 - 1..283369 283369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T65446 WP_038991638.1 NZ_CP016921:104046-104330 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T65446 NZ_CP016921:104046-104330 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT65446 WP_004181777.1 NZ_CP016921:103808-104056 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT65446 NZ_CP016921:103808-104056 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References