Detailed information of TA system TA064961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 349616..350213
• Replicon: chromosome
• Accession: NZ_CP016657
• Organism: Neisseria meningitidis strain M22745

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6L35_RS01725
• Protein ID: WP_002242826.1
• Coordinates: 349616..349894 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6L35_RS01730
• Protein ID: WP_002221020.1
• Coordinates: 349902..350213 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6L35_RS01680	345057..345728	+	672	WP_002217215.1	CsgG/HfaB family protein	-
A6L35_RS01685	345738..346106	+	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6L35_RS01690	346103..346750	+	648	WP_002217218.1	DUF799 domain-containing protein	-
A6L35_RS01705	347192..348335	+	1144	Protein_319	ABC transporter ATP-binding protein/permease	-
A6L35_RS01710	348445..348654	-	210	WP_002222487.1	hypothetical protein	-
A6L35_RS01715	348651..348947	-	297	WP_002221017.1	hypothetical protein	-
A6L35_RS01720	348926..349531	-	606	WP_002219301.1	hypothetical protein	-
A6L35_RS01725	349616..349894	+	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6L35_RS01730	349902..350213	+	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
A6L35_RS01735	350967..352940	-	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6L35_RS01740	352951..353511	-	561	WP_002213590.1	YmfQ family protein	-
A6L35_RS01745	353508..354119	-	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6L35_RS13195	354119..354283	-	165	WP_002224559.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	348651..364510	15859
	-	flank	IS/Tn	-	-	354348..355313	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64961 WP_002242826.1 NZ_CP016657:349616-349894 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64961 NZ_CP016657:349616-349894 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT64961 WP_002221020.1 NZ_CP016657:349902-350213 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64961 NZ_CP016657:349902-350213 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6