Detailed information of TA system TA064897

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3134549..3134774
• Replicon: chromosome
• Accession: NZ_CP016625
• Organism: Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: A9L45_RS16630
• Protein ID: WP_000813254.1
• Coordinates: 3134549..3134704 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3134716..3134774 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9L45_RS16595	3129852..3130910	-	1059	WP_000483497.1	site-specific DNA-methyltransferase	-
A9L45_RS16600	3131061..3131258	-	198	WP_000917735.1	hypothetical protein	-
A9L45_RS16605	3131485..3132306	-	822	WP_000762904.1	antitermination protein	-
A9L45_RS16610	3132303..3132677	-	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
A9L45_RS31905	3132690..3133739	-	1050	Protein_3187	DUF968 domain-containing protein	-
A9L45_RS31910	3133741..3134019	-	279	WP_001304183.1	hypothetical protein	-
A9L45_RS35455	3134085..3134252	-	168	WP_000998188.1	hypothetical protein	-
A9L45_RS16630	3134549..3134704	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3134716..3134774	+	59	-	-	Antitoxin
A9L45_RS33720	3134950..3135054	-	105	WP_001278450.1	hypothetical protein	-
A9L45_RS16635	3135170..3135532	-	363	WP_000610379.1	DUF551 domain-containing protein	-
A9L45_RS16640	3135529..3135900	-	372	WP_000137941.1	hypothetical protein	-
A9L45_RS16645	3135936..3136148	-	213	WP_000063625.1	hypothetical protein	-
A9L45_RS16650	3136197..3136553	-	357	WP_001302146.1	hypothetical protein	-
A9L45_RS16655	3136610..3137005	-	396	WP_001118161.1	DUF977 family protein	-
A9L45_RS16660	3137021..3137791	-	771	WP_000450888.1	DUF1627 domain-containing protein	-
A9L45_RS16665	3137821..3138561	-	741	WP_000790456.1	ATP-binding protein	-
A9L45_RS16670	3138568..3139521	-	954	WP_000095667.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espM1 / nleA/espI / stxB / stxA	3100943..3211002	110059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T64897 WP_000813254.1 NZ_CP016625:c3134704-3134549 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T64897 NZ_CP016625:c3134704-3134549 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT64897 NZ_CP016625:3134716-3134774 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin