Detailed information of TA system TA064685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 358889..359364
• Replicon: chromosome
• Accession: NZ_CP016477
• Organism: Synechococcus sp. PCC 7117

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AWQ22_RS01725
• Protein ID: WP_065709982.1
• Coordinates: 358889..359071 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1B1TV99
• Locus tag: AWQ22_RS01730
• Protein ID: WP_065709983.1
• Coordinates: 359068..359364 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWQ22_RS01705	355183..355401	-	219	WP_065709979.1	hypothetical protein	-
AWQ22_RS01710	355455..356504	-	1050	WP_012305963.1	class 1 fructose-bisphosphatase	-
AWQ22_RS01715	356741..358258	+	1518	WP_065709980.1	4-alpha-glucanotransferase	-
AWQ22_RS01720	358259..358642	+	384	WP_065709981.1	DUF1818 family protein	-
AWQ22_RS16675	358744..358908	+	165	WP_157094565.1	hypothetical protein	-
AWQ22_RS01725	358889..359071	+	183	WP_065709982.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AWQ22_RS01730	359068..359364	+	297	WP_065709983.1	helix-turn-helix domain-containing protein	Antitoxin
AWQ22_RS01735	359477..359695	+	219	WP_030006109.1	hypothetical protein	-
AWQ22_RS01740	360097..360936	+	840	WP_084449026.1	NERD domain-containing protein	-
AWQ22_RS01745	360910..362550	-	1641	WP_065709985.1	rhomboid family intramembrane serine protease	-
AWQ22_RS01750	362634..363563	+	930	WP_065709986.1	putative selenate ABC transporter substrate-binding protein	-
AWQ22_RS01755	363654..364049	-	396	WP_065709987.1	lactoylglutathione lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-54)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-88)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6976.48 Da
• Isoelectric Point: 11.4768

>T64685 WP_065709982.1 NZ_CP016477:358889-359071 [Synechococcus sp. PCC 7117]
MSDGLFELRIKGREGIARVFYCTLVGKRIVMLHGFVKKSQKTPAKELRIAKRRMLEVKNK

Nucleotide

• Length: 183 bp

>T64685 NZ_CP016477:358889-359071 [Synechococcus sp. PCC 7117]
ATGTCAGATGGCTTATTTGAACTTCGCATTAAAGGTAGAGAAGGGATTGCACGAGTGTTTTACTGCACGCTAGTAGGGAA
ACGGATCGTTATGCTGCATGGCTTTGTGAAAAAATCTCAGAAAACACCAGCGAAGGAACTCAGAATTGCCAAACGTCGTA
TGTTGGAGGTCAAGAATAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10859.53 Da
• Isoelectric Point: 9.6868

>AT64685 WP_065709983.1 NZ_CP016477:359068-359364 [Synechococcus sp. PCC 7117]
MNHTELKKKALANPETRIMYDDMEAEFSLLRQMLKARQEAGLSQAEVAARMGTKAPAITRLESSLSNGKHSPSLATLRRY
AQAVGCELQVTLIKSENI

Nucleotide

• Length: 297 bp

>AT64685 NZ_CP016477:359068-359364 [Synechococcus sp. PCC 7117]
ATGAATCATACTGAACTGAAAAAAAAGGCATTAGCTAATCCTGAAACGCGTATTATGTACGACGACATGGAAGCTGAATT
TTCATTGTTGAGACAAATGCTCAAAGCAAGGCAAGAAGCTGGTCTTTCTCAAGCGGAGGTTGCTGCTCGCATGGGAACTA
AAGCCCCCGCTATCACACGGTTAGAATCATCTCTTAGTAATGGCAAGCATTCACCTTCTTTGGCAACCCTACGCCGTTAT
GCTCAGGCTGTAGGTTGTGAGCTTCAGGTAACATTAATTAAGTCTGAAAATATTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1B1TV99