Detailed information of TA system TA064380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 66945..67603
• Replicon: plasmid pCMCVTAb2-Ab4
• Accession: NZ_CP016297
• Organism: Acinetobacter baumannii strain CMC-CR-MDR-Ab4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AOT16_RS20260
• Protein ID: WP_000312250.1
• Coordinates: 67244..67603 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOT16_RS20255
• Protein ID: WP_001096429.1
• Coordinates: 66945..67244 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOT16_RS20210	62797..63249	+	453	Protein_73	hypothetical protein	-
AOT16_RS20215	63354..63611	+	258	WP_000834290.1	hypothetical protein	-
AOT16_RS20220	63616..64188	+	573	WP_000443897.1	hypothetical protein	-
AOT16_RS20225	64164..64343	+	180	WP_000387630.1	hypothetical protein	-
AOT16_RS20230	64360..64989	+	630	WP_000701004.1	hypothetical protein	-
AOT16_RS20235	65029..65247	+	219	WP_001043201.1	hypothetical protein	-
AOT16_RS20240	65267..65821	+	555	WP_000790084.1	hypothetical protein	-
AOT16_RS20245	65871..66407	+	537	WP_000731978.1	hypothetical protein	-
AOT16_RS20255	66945..67244	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
AOT16_RS20260	67244..67603	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOT16_RS20265	67804..68370	+	567	WP_000710385.1	hypothetical protein	-
AOT16_RS20270	68419..68601	+	183	WP_000373385.1	hypothetical protein	-
AOT16_RS20275	68668..69366	+	699	WP_000873188.1	hypothetical protein	-
AOT16_RS20280	69505..69888	+	384	WP_000654348.1	hypothetical protein	-
AOT16_RS20285	69955..70713	+	759	WP_001053127.1	hypothetical protein	-
AOT16_RS20295	71768..72082	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / blaOXA-23	-	1..74090	74090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T64380 WP_000312250.1 NZ_CP016297:c67603-67244 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T64380 NZ_CP016297:c67603-67244 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT64380 WP_001096429.1 NZ_CP016297:c67244-66945 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT64380 NZ_CP016297:c67244-66945 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA