Detailed information of TA system TA064100

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1670630..1671462
• Replicon: chromosome
• Accession: NZ_CP016007
• Organism: Escherichia coli strain NGF1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: A9Z04_RS07965
• Protein ID: WP_000854753.1
• Coordinates: 1671088..1671462 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: A9Z04_RS07960
• Protein ID: WP_001278232.1
• Coordinates: 1670630..1670998 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9Z04_RS07925	1667466..1667921	+	456	WP_000581504.1	hypothetical protein	-
A9Z04_RS07930	1668000..1668233	+	234	WP_001119729.1	DUF905 family protein	-
A9Z04_RS07935	1668333..1669151	+	819	WP_001520163.1	DUF945 domain-containing protein	-
A9Z04_RS07940	1669206..1669691	+	486	WP_001520165.1	antirestriction protein	-
A9Z04_RS07945	1669707..1670183	+	477	WP_001186726.1	RadC family protein	-
A9Z04_RS07950	1670246..1670467	+	222	WP_000692316.1	DUF987 domain-containing protein	-
A9Z04_RS07960	1670630..1670998	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
A9Z04_RS07965	1671088..1671462	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
A9Z04_RS07970	1671459..1671947	+	489	WP_000777541.1	hypothetical protein	-
A9Z04_RS07975	1671959..1672156	+	198	WP_000839281.1	DUF957 domain-containing protein	-
A9Z04_RS07980	1672253..1672822	+	570	WP_001290241.1	DUF4942 domain-containing protein	-
A9Z04_RS07985	1673164..1673334	+	171	Protein_1525	IS110 family transposase	-
A9Z04_RS07990	1674145..1675128	+	984	WP_001616958.1	KpsF/GutQ family sugar-phosphate isomerase	-
A9Z04_RS07995	1675200..1676348	+	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T64100 WP_000854753.1 NZ_CP016007:1671088-1671462 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T64100 NZ_CP016007:1671088-1671462 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT64100 WP_001278232.1 NZ_CP016007:1670630-1670998 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT64100 NZ_CP016007:1670630-1670998 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72