Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 2694901..2696144 Replicon chromosome
Accession NZ_CP015950
Organism Legionella pneumophila strain E4_N

Toxin (Protein)


Gene name HipT Uniprot ID Q5ZSZ6
Locus tag A9E91_RS11890 Protein ID WP_010948074.1
Coordinates 2695206..2696144 (+) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5ZSZ7
Locus tag A9E91_RS11885 Protein ID WP_010948073.1
Coordinates 2694901..2695209 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E91_RS11860 2690337..2690702 + 366 WP_010948069.1 BRCT domain-containing protein -
A9E91_RS11865 2691059..2691424 - 366 WP_010948070.1 TraK family protein -
A9E91_RS15730 2691609..2691749 - 141 WP_153802426.1 hypothetical protein -
A9E91_RS11870 2691752..2693503 - 1752 WP_010948071.1 DUF927 domain-containing protein -
A9E91_RS11875 2693569..2693844 - 276 WP_010948072.1 helix-turn-helix domain-containing protein -
A9E91_RS11880 2694677..2694904 + 228 WP_006870829.1 helix-turn-helix transcriptional regulator -
A9E91_RS11885 2694901..2695209 + 309 WP_010948073.1 HipA N-terminal domain-containing protein Antitoxin
A9E91_RS11890 2695206..2696144 + 939 WP_010948074.1 lpg2370 family Dot/Icm T4SS effector Toxin
A9E91_RS11895 2696687..2697301 + 615 WP_010948075.1 hypothetical protein -
A9E91_RS11900 2697457..2697663 - 207 WP_015444115.1 hypothetical protein -
A9E91_RS11905 2698000..2699268 + 1269 WP_010948076.1 lpg2372 family Dot/Icm T4SS effector -
A9E91_RS11910 2699484..2700009 - 526 Protein_2342 hypothetical protein -
A9E91_RS11915 2700009..2700674 - 666 WP_015444114.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2688923..2690098 1175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-276)

Antitoxin

HipA, N-terminal subdomain 1

(5-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36077.81 Da        Isoelectric Point: 8.6882

>T63976 WP_010948074.1 NZ_CP015950:2695206-2696144 [Legionella pneumophila]
MKHCPITYEKISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSVQGVQKKLSAKLKIKEGCFEIVDQYG
QYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLLNSTIAQKNTKEELALPLKG
KKNNLTKSDFLKYFAIEKLGLNQNVIDGIVQEFHQVIPKWQELIGFSFLSQEMQEKYLELLEQRCKRLNFFD

Download         Length: 939 bp

>T63976 NZ_CP015950:2695206-2696144 [Legionella pneumophila]
ATGAAACACTGCCCTATTACTTATGAAAAGATTAGTGACCAGGAAAACTATTCGCAACGTGGATTGCATTTGCTTTCTCC
TCAATTAAAAAACCTTAGTCCATTGGATTTAAGTGCTGATGAGCAGCGTCAGGAAGCAATTGCTCGAGTAGGAAAGATGT
CTGTTCAAGGCGTCCAAAAGAAACTAAGCGCAAAACTAAAGATTAAAGAAGGCTGTTTTGAAATCGTGGATCAATATGGT
CAGTATATTTTAAAACCACAAAGTGATATTTATCCAGAATTGCCTGAAAATGAAGCCATTACTATGACCCTTGCAAAAAC
TATAGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTAACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACAAAATATAAAAGT
TCTATGGAAAAAGTAATTGCAGTCATAGAACAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGTTGAC
GTTGTTTAACTTCTTGGTCGGTAATGAAGACATGCATCTAAAAAACTTTTCCTTAATTACGAAGGATAGAAAAATTTCCA
TATCACCAGCCTACGATTTACTTAACTCAACCATCGCTCAAAAAAACACAAAAGAAGAACTGGCTTTACCTCTAAAAGGA
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCATTGAAAAATTAGGATTAAACCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAGGTAATACCAAAATGGCAAGAGCTGATTGGCTTCAGTTTTTTATCTCAAGAGATGC
AGGAAAAATATCTCGAATTGCTAGAGCAACGATGCAAGCGATTAAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11557.38 Da        Isoelectric Point: 4.5994

>AT63976 WP_010948073.1 NZ_CP015950:2694901-2695209 [Legionella pneumophila]
MRKAYVSVSGIKAGILEELQGGTYQFTYFEDYHGAPVSLTMPLKNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLILVGQDVVGAVTIEEIR

Download         Length: 309 bp

>AT63976 NZ_CP015950:2694901-2695209 [Legionella pneumophila]
ATGAGAAAAGCATACGTATCGGTGAGTGGCATTAAGGCTGGAATATTAGAAGAATTACAAGGTGGAACCTATCAATTTAC
CTACTTTGAGGATTATCACGGCGCACCTGTTTCACTCACTATGCCGTTGAAAAATAAGGTCTATGATTTTGACGTGTTCC
CCCCTTTTTTTGAAGGATTACTTCCTGAAGGCATTATGCTTGAAGCACTATTACGCAAATACAAGATAGATAAAAATGAT
TATTTTGGTCAATTGATACTAGTTGGCCAAGATGTCGTAGGCGCAGTGACTATTGAGGAAATAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VKC
PDB 7VKB
PDB 7WCF

Antitoxin

Source ID Structure
PDB 7VKB
AlphaFold DB Q5ZSZ7

References