Detailed information of TA system TA063972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 60966..61564
• Replicon: plasmid unnamed1
• Accession: NZ_CP015948
• Organism: Legionella pneumophila strain E2_N

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: A9E89_RS15195
• Protein ID: WP_058475085.1
• Coordinates: 60966..61163 (+)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378P8P3
• Locus tag: A9E89_RS15200
• Protein ID: WP_058475084.1
• Coordinates: 61160..61564 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E89_RS15180	58056..59420	+	1365	WP_061363916.1	hypothetical protein	-
A9E89_RS15185	59438..59899	-	462	WP_061363915.1	WGR domain-containing protein	-
A9E89_RS15190	59899..60783	-	885	WP_061363914.1	recombinase family protein	-
A9E89_RS15195	60966..61163	+	198	WP_058475085.1	type II toxin-antitoxin system HicA family toxin	Toxin
A9E89_RS15200	61160..61564	+	405	WP_058475084.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A9E89_RS15205	61589..63283	+	1695	WP_061363913.1	hypothetical protein	-
A9E89_RS15210	64296..64586	-	291	WP_021460513.1	helix-turn-helix domain-containing protein	-
A9E89_RS15215	64564..64917	-	354	WP_061363912.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A9E89_RS15800	64956..65318	-	363	WP_129462639.1	hypothetical protein	-
A9E89_RS15805	65376..65486	+	111	WP_158513232.1	(2Fe-2S)-binding protein	-
A9E89_RS15225	65827..66150	-	324	WP_061363910.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71453	71453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7389.76 Da
• Isoelectric Point: 10.7302

>T63972 WP_058475085.1 NZ_CP015948:60966-61163 [Legionella pneumophila]
MKSAEIIKIIERDGWVKCHQKGSHCQFKHPTKKGRVTVPHPKKDLPVKTVASIFKQAGINKEDIK

Nucleotide

• Length: 198 bp

>T63972 NZ_CP015948:60966-61163 [Legionella pneumophila]
ATGAAAAGCGCAGAAATAATAAAAATAATAGAACGCGATGGTTGGGTGAAGTGTCACCAGAAAGGGAGTCATTGCCAATT
TAAGCATCCAACAAAAAAGGGTAGGGTGACTGTGCCTCATCCTAAAAAAGATTTACCCGTTAAAACGGTAGCTAGTATTT
TTAAACAGGCTGGAATTAATAAGGAGGATATAAAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14961.80 Da
• Isoelectric Point: 4.5209

>AT63972 WP_058475084.1 NZ_CP015948:61160-61564 [Legionella pneumophila]
MRYPVVIHKDEHSDYGVSVPDIPGCYSAGATYDEALTNVIEAIECHLEGLLMDNESIPVGSTIDHWINEEEFQGGVWAFI
DIDLSQISGKSKRINITIPERVLNLIDLYSKNHAVKNRSSFLADAALSYIESHK

Nucleotide

• Length: 405 bp

>AT63972 NZ_CP015948:61160-61564 [Legionella pneumophila]
ATGAGATATCCTGTAGTAATACATAAAGACGAGCACAGTGATTATGGCGTAAGTGTCCCAGATATTCCTGGATGTTATTC
TGCTGGAGCTACTTATGATGAGGCGTTAACTAACGTCATTGAGGCAATTGAATGTCATTTAGAAGGGTTGTTAATGGACA
ATGAGTCCATACCAGTAGGTTCTACAATTGATCATTGGATAAATGAGGAAGAGTTTCAGGGTGGAGTGTGGGCATTTATT
GATATTGATTTAAGTCAAATATCAGGTAAATCAAAACGCATCAATATCACTATTCCAGAGCGCGTATTAAATCTTATTGA
TTTATATTCTAAAAATCATGCAGTAAAAAATCGCTCTTCATTTTTAGCAGATGCAGCACTTAGTTATATAGAAAGTCATA
AATAA

Structures

Antitoxin

• AlphaFold DB: A0A378P8P3