Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 75991..76516 Replicon plasmid unnamed
Accession NZ_CP015854
Organism Escherichia coli strain ATCC 43889

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A8714_RS28885 Protein ID WP_001159868.1
Coordinates 76211..76516 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A8714_RS28880 Protein ID WP_000813634.1
Coordinates 75991..76209 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8714_RS28845 71274..71504 + 231 WP_010891288.1 hypothetical protein -
A8714_RS28850 71625..72365 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
A8714_RS28855 72650..73627 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
A8714_RS28865 74035..74235 - 201 WP_000708307.1 hypothetical protein -
A8714_RS28870 74232..74852 - 621 WP_001248529.1 hypothetical protein -
A8714_RS28875 74849..75532 - 684 WP_010891291.1 DNA-binding protein -
A8714_RS28880 75991..76209 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A8714_RS28885 76211..76516 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A8714_RS28890 76517..77323 + 807 WP_000016989.1 site-specific integrase -
A8714_RS28905 79313..79675 + 363 Protein_77 RepB family plasmid replication initiator protein -
A8714_RS28910 80263..81429 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / hlyD 1..92854 92854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T63820 WP_001159868.1 NZ_CP015854:76211-76516 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T63820 NZ_CP015854:76211-76516 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT63820 WP_000813634.1 NZ_CP015854:75991-76209 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT63820 NZ_CP015854:75991-76209 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References