Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1487394..1488189 Replicon chromosome
Accession NZ_CP015846
Organism Escherichia coli O157:H7 strain FRIK2069

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag A8V30_RS07785 Protein ID WP_000854914.1
Coordinates 1487815..1488189 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LM11
Locus tag A8V30_RS07780 Protein ID WP_001280953.1
Coordinates 1487394..1487768 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8V30_RS07735 1482749..1483654 + 906 WP_000203541.1 chemotaxis protein -
A8V30_RS07740 1483651..1484721 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
A8V30_RS07755 1485061..1485879 + 819 WP_001234682.1 DUF945 domain-containing protein -
A8V30_RS07760 1485970..1486455 + 486 WP_000214398.1 antirestriction protein -
A8V30_RS07765 1486471..1486947 + 477 WP_001186738.1 RadC family protein -
A8V30_RS07770 1487010..1487231 + 222 WP_000692323.1 DUF987 domain-containing protein -
A8V30_RS07780 1487394..1487768 + 375 WP_001280953.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
A8V30_RS07785 1487815..1488189 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
A8V30_RS07790 1488186..1488677 + 492 WP_000976857.1 hypothetical protein -
A8V30_RS07795 1488689..1488886 + 198 WP_000839282.1 DUF957 domain-containing protein -
A8V30_RS07800 1488971..1489813 + 843 WP_001280481.1 DUF4942 domain-containing protein -
A8V30_RS07810 1490284..1491222 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
A8V30_RS07815 1491277..1492014 + 738 WP_000283664.1 zinc-binding phosphatase -
A8V30_RS07820 1492038..1492592 + 555 WP_001001917.1 molecular chaperone YcdY -
A8V30_RS07825 1492694..1493185 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1486471..1498753 12282


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T63738 WP_000854914.1 NZ_CP015846:1487815-1488189 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T63738 NZ_CP015846:1487815-1488189 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13734.56 Da        Isoelectric Point: 6.6249

>AT63738 WP_001280953.1 NZ_CP015846:1487394-1487768 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Download         Length: 375 bp

>AT63738 NZ_CP015846:1487394-1487768 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LM11

References