Detailed information of TA system TA063639

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4724..4988
• Replicon: plasmid pMS6198D
• Accession: NZ_CP015838
• Organism: Escherichia coli strain MS6198

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: MS6198_RS27775
• Protein ID: WP_001387489.1
• Coordinates: 4836..4988 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	4724..4784 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6198_RS27745	849..1112	+	264	WP_032180564.1	ash family protein	-
MS6198_RS27750	1030..1317	+	288	WP_000074855.1	conjugal transfer protein TraA	-
MS6198_RS27755	1759..2064	+	306	Protein_2	transcription termination factor NusG	-
MS6198_RS27760	2078..2775	-	698	Protein_3	IS1 family transposase	-
MS6198_RS27765	2831..3988	-	1158	Protein_4	IS1380-like element ISEc9 family transposase	-
MS6198_RS31385	4174..4521	-	348	Protein_5	protein finQ	-
-	4724..4784	-	61	NuclAT_0	-	Antitoxin
-	4724..4784	-	61	NuclAT_0	-	Antitoxin
-	4724..4784	-	61	NuclAT_0	-	Antitoxin
-	4724..4784	-	61	NuclAT_0	-	Antitoxin
MS6198_RS27775	4836..4988	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
MS6198_RS27780	5060..5311	-	252	WP_001291964.1	hypothetical protein	-
MS6198_RS27785	5611..5907	+	297	WP_011264046.1	hypothetical protein	-
MS6198_RS31625	5972..6148	-	177	WP_001054897.1	hypothetical protein	-
MS6198_RS27800	6540..6749	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
MS6198_RS27805	6974..7671	+	698	Protein_11	IS1 family transposase	-
MS6198_RS27815	8124..9268	+	1145	Protein_12	CMY-2 family class C beta-lactamase	-
MS6198_RS27820	9362..9895	+	534	WP_001221666.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..50899	50899
	-	inside	IScluster/Tn	blaCMY-42	-	2500..9268	6768

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T63639 WP_001387489.1 NZ_CP015838:4836-4988 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T63639 NZ_CP015838:4836-4988 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT63639 NZ_CP015838:c4784-4724 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin