Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 36099..36658 Replicon plasmid p0157
Accession NZ_CP015816
Organism Escherichia coli O157:H7 strain JEONG-1266

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag JEONG1266_RS28300 Protein ID WP_000421248.1
Coordinates 36099..36374 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JEONG1266_RS28305 Protein ID WP_001178089.1
Coordinates 36374..36658 (-) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JEONG1266_RS28265 31452..31841 - 390 WP_001172748.1 cytochrome b562 family protein -
JEONG1266_RS28270 31885..34095 - 2211 WP_000592771.1 catalase/peroxidase KatP -
JEONG1266_RS28275 34272..34457 + 186 WP_001302179.1 hypothetical protein -
JEONG1266_RS28280 34457..34873 + 417 Protein_30 IS91 family transposase -
JEONG1266_RS30940 34873..35439 + 567 Protein_31 IS3-like element IS600 family transposase -
JEONG1266_RS28290 35483..35698 + 216 Protein_32 transposase -
JEONG1266_RS28295 35798..36004 - 207 WP_001105064.1 hypothetical protein -
JEONG1266_RS28300 36099..36374 - 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JEONG1266_RS28305 36374..36658 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JEONG1266_RS28310 37570..38427 - 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
JEONG1266_RS28315 38420..38494 - 75 WP_001370046.1 RepA leader peptide Tap -
JEONG1266_RS28320 38730..38984 - 255 WP_000083831.1 replication regulatory protein RepA -
JEONG1266_RS28325 39224..39562 - 339 WP_000766796.1 hypothetical protein -
JEONG1266_RS28330 39600..39809 - 210 WP_001302200.1 hemolysin expression modulator Hha -
JEONG1266_RS28335 39855..40316 - 462 WP_001233856.1 thermonuclease family protein -
JEONG1266_RS28340 40561..40773 - 213 WP_001302189.1 ANR family transcriptional regulator -
JEONG1266_RS28345 40906..41466 - 561 WP_000840472.1 fertility inhibition protein FinO -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - exeG / exeE / stcE / espP / toxB / hlyD / hlyB / hlyA / hlyC 1..95910 95910


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T63479 WP_000421248.1 NZ_CP015816:c36374-36099 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T63479 NZ_CP015816:c36374-36099 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT63479 WP_001178089.1 NZ_CP015816:c36658-36374 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT63479 NZ_CP015816:c36658-36374 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0


Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References