Detailed information of TA system TA063335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2299045..2299712
• Replicon: chromosome
• Accession: NZ_CP015749
• Organism: Pectobacterium parmentieri strain RNS 08-42-1A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3F7E4
• Locus tag: A8F97_RS10335
• Protein ID: WP_033071239.1
• Coordinates: 2299398..2299712 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A8F97_RS10330
• Protein ID: WP_015841345.1
• Coordinates: 2299045..2299386 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8F97_RS10285	2294308..2295423	+	1116	WP_033071248.1	phage major capsid protein, P2 family	-
A8F97_RS10290	2295427..2296086	+	660	WP_033071247.1	terminase endonuclease subunit	-
A8F97_RS10295	2296178..2296681	+	504	WP_033071246.1	head completion/stabilization protein	-
A8F97_RS10300	2296681..2296884	+	204	WP_033071245.1	tail protein X	-
A8F97_RS10305	2296875..2297096	+	222	WP_033071244.1	hypothetical protein	-
A8F97_RS10310	2297080..2297589	+	510	WP_033071243.1	lysozyme	-
A8F97_RS10315	2297586..2298029	+	444	WP_033071242.1	Rz-like lysis system protein LysB	-
A8F97_RS10320	2298119..2298574	+	456	WP_033071241.1	phage tail protein	-
A8F97_RS10325	2298567..2299016	+	450	WP_033071240.1	phage virion morphogenesis protein	-
A8F97_RS10330	2299045..2299386	-	342	WP_015841345.1	HigA family addiction module antidote protein	Antitoxin
A8F97_RS10335	2299398..2299712	-	315	WP_033071239.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8F97_RS10340	2300056..2300685	+	630	WP_033071238.1	ORF6N domain-containing protein	-
A8F97_RS10345	2300731..2301480	-	750	WP_127087869.1	hypothetical protein	-
A8F97_RS10350	2301587..2302222	+	636	WP_033071236.1	phage baseplate assembly protein V	-
A8F97_RS10355	2302219..2302563	+	345	WP_033071235.1	GPW/gp25 family protein	-
A8F97_RS10360	2302568..2303476	+	909	WP_033071234.1	baseplate assembly protein	-
A8F97_RS10365	2303469..2304080	+	612	WP_033071233.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12183.99 Da
• Isoelectric Point: 10.1205

>T63335 WP_033071239.1 NZ_CP015749:c2299712-2299398 [Pectobacterium parmentieri]
MQKMRSIHSFRDKWLEDFFMYGKSHKKIPADVVTSLSRKLDIINAAVSAKDLRSPPGNRYENLNPPLGEYSSIRVNIQYR
LIFKWVDGKAIDVYLDDHGYKNHK

Nucleotide

• Length: 315 bp

>T63335 NZ_CP015749:c2299712-2299398 [Pectobacterium parmentieri]
ATGCAAAAAATGCGCAGCATTCATAGCTTTCGGGATAAGTGGCTGGAAGATTTTTTTATGTATGGTAAGTCGCATAAAAA
GATACCTGCCGATGTGGTTACATCCTTGTCCCGCAAGCTGGATATCATCAACGCGGCGGTATCGGCAAAAGATCTGAGAT
CGCCACCGGGGAACCGATACGAGAATCTCAACCCACCATTGGGGGAATACTCATCCATCAGAGTGAATATCCAATATCGC
CTGATTTTTAAATGGGTTGATGGTAAAGCGATTGATGTTTATCTGGATGACCACGGTTACAAAAATCATAAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12941.56 Da
• Isoelectric Point: 4.4346

>AT63335 WP_015841345.1 NZ_CP015749:c2299386-2299045 [Pectobacterium parmentieri]
MAQAQRKPTSVGDVLLYEYLEPTGLKINDLAEMLNVHRNTISALVNNNRKLTVDMAFRLAKAFDTSVDFWLNLQNSVDVW
EVENDARTQEEISRITTLENFLAQRDENVKDVA

Nucleotide

• Length: 342 bp

>AT63335 NZ_CP015749:c2299386-2299045 [Pectobacterium parmentieri]
ATGGCACAGGCACAACGCAAGCCGACATCCGTAGGCGATGTTCTGTTGTATGAATATCTTGAACCGACGGGGTTGAAGAT
TAATGACCTGGCGGAAATGCTGAACGTCCACCGCAATACGATCAGCGCATTGGTCAACAACAACCGTAAGCTAACGGTTG
ATATGGCTTTCCGGTTGGCGAAAGCGTTTGATACATCAGTTGATTTCTGGCTTAACCTGCAAAATAGTGTTGACGTATGG
GAAGTGGAAAATGACGCGCGCACTCAGGAAGAAATTAGCCGGATCACCACGCTGGAAAATTTTCTGGCGCAGCGCGATGA
AAATGTGAAAGACGTCGCATAA

Structures

Toxin

• AlphaFold DB: A0A8B3F7E4