Detailed information of TA system TA063332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 230704..231209
• Replicon: plasmid pShin-03
• Accession: NZ_CP015739
• Organism: Shinella sp. HZN7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: shn_RS28280
• Protein ID: WP_084438610.1
• Coordinates: 230955..231209 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1A9GB25
• Locus tag: shn_RS28275
• Protein ID: WP_064333867.1
• Coordinates: 230704..230958 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
shn_RS28255	226836..227522	-	687	WP_064333863.1	ATP-binding cassette domain-containing protein	-
shn_RS28260	227525..227989	-	465	WP_064333864.1	hypothetical protein	-
shn_RS28265	228062..229816	-	1755	WP_064333865.1	alkaline phosphatase	-
shn_RS28270	229925..230539	-	615	WP_064333866.1	hypothetical protein	-
shn_RS28275	230704..230958	+	255	WP_064333867.1	hypothetical protein	Antitoxin
shn_RS28280	230955..231209	+	255	WP_084438610.1	PIN domain-containing protein	Toxin
shn_RS28285	231223..232128	-	906	WP_064333868.1	LysR family transcriptional regulator	-
shn_RS28290	232255..232734	+	480	WP_064333869.1	carboxymuconolactone decarboxylase family protein	-
shn_RS28295	232824..233240	+	417	WP_064333870.1	cupin domain-containing protein	-
shn_RS28300	233292..233780	-	489	WP_064334041.1	Rrf2 family transcriptional regulator	-
shn_RS28305	233860..234960	-	1101	WP_064333871.1	NAD(P)/FAD-dependent oxidoreductase	-
shn_RS28310	235053..235982	-	930	WP_064333872.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..409126	409126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-60)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 8872.41 Da
• Isoelectric Point: 4.6321

>T63332 WP_084438610.1 NZ_CP015739:230955-231209 [Shinella sp. HZN7]
MIILDTSVVSEAMKPIPDDAVRSWLDEQAVETLFLTSVTVAELMFGIGALPAGKRREKLSAALDGLMTVIPAASKALVSR
VATS

Nucleotide

• Length: 255 bp

>T63332 NZ_CP015739:230955-231209 [Shinella sp. HZN7]
ATGATCATTCTCGATACGAGTGTCGTCTCCGAGGCCATGAAGCCCATCCCCGACGATGCCGTGCGTTCCTGGCTGGACGA
GCAGGCCGTCGAAACGCTTTTCCTCACCAGCGTGACGGTCGCGGAATTGATGTTCGGCATCGGTGCCCTGCCGGCGGGCA
AGCGCCGAGAAAAACTTTCCGCCGCGCTGGATGGATTGATGACGGTGATACCCGCCGCATCGAAGGCACTGGTATCGCGG
GTTGCAACGTCGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9188.39 Da
• Isoelectric Point: 7.2060

>AT63332 WP_064333867.1 NZ_CP015739:230704-230958 [Shinella sp. HZN7]
MPAVTIRNLSEATHRALKVRAAQHGRSTEAEMRDILEAAVRPQARLRLGSALAERSRSLGLTNEDFAALDEARDTLPATT
MGFE

Nucleotide

• Length: 255 bp

>AT63332 NZ_CP015739:230704-230958 [Shinella sp. HZN7]
ATGCCGGCGGTTACAATCCGCAACCTTTCCGAGGCGACGCATCGTGCCTTGAAAGTCCGCGCGGCGCAGCATGGGCGCAG
CACGGAGGCGGAGATGCGCGACATTCTCGAGGCCGCGGTCCGCCCGCAAGCGCGGCTGCGGCTGGGCAGCGCGCTGGCCG
AGCGCAGCCGCAGCCTCGGCCTGACGAACGAGGATTTCGCCGCGCTCGATGAGGCCCGCGACACGCTGCCGGCAACGACG
ATGGGCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1A9GB25