Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 432466..433052 Replicon plasmid pShin-02
Accession NZ_CP015738
Organism Shinella sp. HZN7

Toxin (Protein)


Gene name vapC Uniprot ID A0A1A9GBT6
Locus tag shn_RS27140 Protein ID WP_064333634.1
Coordinates 432466..432855 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1A9GBF2
Locus tag shn_RS27145 Protein ID WP_064333635.1
Coordinates 432852..433052 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
shn_RS27125 428683..430107 + 1425 WP_064333631.1 RtcB family protein -
shn_RS27130 430339..431556 + 1218 WP_064333632.1 RNA ligase RtcB family protein -
shn_RS27135 431543..432154 + 612 WP_064333633.1 peptide chain release factor H -
shn_RS27140 432466..432855 - 390 WP_064333634.1 type II toxin-antitoxin system VapC family toxin Toxin
shn_RS27145 432852..433052 - 201 WP_064333635.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
shn_RS27150 433200..434027 - 828 WP_064333636.1 transglutaminase family protein -
shn_RS27155 434105..435001 - 897 WP_064333637.1 transglutaminase family protein -
shn_RS27160 435158..435373 - 216 WP_064333680.1 helix-turn-helix domain-containing protein -
shn_RS27165 435788..437350 + 1563 WP_168168977.1 hybrid sensor histidine kinase/response regulator -
shn_RS27170 437355..437987 + 633 WP_064333639.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - adeG 1..445803 445803


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-112)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14538.72 Da        Isoelectric Point: 7.1429

>T63331 WP_064333634.1 NZ_CP015738:c432855-432466 [Shinella sp. HZN7]
VILVDTSIWIDHFRHGDAELRKIIEDDRLLYHPVIIGELALGSLRDRDAVMAFLSAQRQAFVATHDEVMTMIDRHSIFSM
GIGYMDAHLLASTLLDRRSLLWTRDKRLAAAAQKAGAPLHTPTSSSNRF

Download         Length: 390 bp

>T63331 NZ_CP015738:c432855-432466 [Shinella sp. HZN7]
GTGATCCTGGTAGATACCTCAATTTGGATTGATCATTTTCGCCATGGCGATGCAGAGTTACGCAAAATCATCGAGGACGA
CCGCCTACTCTACCACCCCGTCATTATCGGCGAGCTGGCGCTCGGCAGTCTGCGGGACCGTGATGCCGTGATGGCCTTCC
TGTCGGCACAGCGCCAAGCATTCGTCGCCACGCACGATGAGGTCATGACGATGATTGATCGGCATTCAATTTTCAGCATG
GGGATCGGCTACATGGACGCCCATTTGTTGGCCTCTACGCTACTTGACCGGCGATCGCTCTTGTGGACGAGAGACAAACG
TCTCGCAGCCGCGGCTCAAAAGGCAGGCGCCCCACTGCACACACCTACGAGCTCATCCAACCGATTCTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7209.33 Da        Isoelectric Point: 11.0968

>AT63331 WP_064333635.1 NZ_CP015738:c433052-432852 [Shinella sp. HZN7]
MRSTINLDDKLMERAKALTGTRETAALVRQALETLVRVESGKRLIALGGSMPDAEAAPRRRSEATK

Download         Length: 201 bp

>AT63331 NZ_CP015738:c433052-432852 [Shinella sp. HZN7]
ATGCGATCTACTATCAATCTCGACGACAAGCTTATGGAGCGAGCAAAAGCGCTAACCGGGACCAGGGAAACGGCTGCGCT
TGTCCGCCAGGCATTGGAGACCCTTGTCAGGGTCGAATCCGGAAAGCGGCTCATCGCGCTCGGGGGCTCGATGCCCGATG
CCGAAGCAGCCCCGCGCCGTCGGAGCGAAGCGACGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A9GBT6

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9GBF2

References