Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 2701182..2701773 Replicon chromosome
Accession NZ_CP015736
Organism Shinella sp. HZN7

Toxin (Protein)


Gene name PumA Uniprot ID A0A1A9G108
Locus tag shn_RS12990 Protein ID WP_064330773.1
Coordinates 2701182..2701481 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1A9G2U9
Locus tag shn_RS12995 Protein ID WP_064330774.1
Coordinates 2701483..2701773 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
shn_RS12975 2697076..2698344 - 1269 WP_064330770.1 cytochrome b N-terminal domain-containing protein -
shn_RS12980 2698359..2698937 - 579 WP_084437973.1 ubiquinol-cytochrome c reductase iron-sulfur subunit -
shn_RS12985 2699202..2701070 - 1869 WP_064330772.1 ABC transporter ATP-binding protein/permease -
shn_RS12990 2701182..2701481 + 300 WP_064330773.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
shn_RS12995 2701483..2701773 + 291 WP_064330774.1 putative addiction module antidote protein Antitoxin
shn_RS13000 2701813..2703666 - 1854 WP_064330775.1 ABC transporter ATP-binding protein/permease -
shn_RS13005 2704118..2704579 + 462 WP_064330776.1 tRNA (cytidine(34)-2'-O)-methyltransferase -
shn_RS13010 2704656..2705864 - 1209 WP_084437978.1 MFS transporter -
shn_RS13015 2705861..2706433 - 573 WP_064330777.1 NAD(P)H-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2699202..2715640 16438


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-97)

Antitoxin

Uncharacterized protein HI1420

(7-92)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 10952.69 Da        Isoelectric Point: 10.5786

>T63329 WP_064330773.1 NZ_CP015736:2701182-2701481 [Shinella sp. HZN7]
MTLLIKQTDTFRKWERALGDRKARALIAARLNRIAYGLIGDVKSVGDGVSEIRIHHGPGYRIYFTRRGEEIIVLLCGGDK
GSQKRDIAAAKTLAAQLDS

Download         Length: 300 bp

>T63329 NZ_CP015736:2701182-2701481 [Shinella sp. HZN7]
ATGACGCTCCTGATCAAGCAGACGGATACATTTCGCAAATGGGAGCGGGCGCTCGGTGATCGCAAAGCACGCGCGCTGAT
CGCCGCCCGGCTGAACCGGATCGCCTACGGCCTTATCGGCGACGTCAAATCCGTCGGTGACGGCGTCAGCGAAATCCGCA
TCCACCATGGACCCGGATATCGCATCTATTTCACGCGAAGGGGCGAAGAAATCATCGTGCTGCTCTGCGGCGGCGACAAG
GGTTCCCAGAAACGGGATATCGCCGCCGCCAAGACCCTGGCAGCCCAACTGGACAGCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10234.64 Da        Isoelectric Point: 5.6863

>AT63329 WP_064330774.1 NZ_CP015736:2701483-2701773 [Shinella sp. HZN7]
MNKTLHTYDPAEALTSDEAVDVFMKDAFETGDAGYIAHALGVVARARGMSKVAEETGLAREALYRSLASDGNPTLKSMLT
VMRALGLELTSKRAGT

Download         Length: 291 bp

>AT63329 NZ_CP015736:2701483-2701773 [Shinella sp. HZN7]
ATGAACAAAACATTGCACACCTATGATCCGGCAGAGGCTTTGACCTCCGACGAAGCCGTCGATGTCTTCATGAAGGATGC
GTTCGAGACGGGCGATGCGGGTTATATCGCGCATGCCCTCGGCGTCGTCGCGCGGGCCAGGGGCATGAGCAAGGTCGCGG
AAGAAACCGGCCTCGCCCGGGAAGCGCTCTACCGCTCGCTTGCCAGCGACGGCAATCCGACCCTCAAGTCCATGCTGACG
GTCATGCGCGCCCTCGGACTTGAGCTGACGTCAAAGCGCGCCGGCACTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A9G108

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9G2U9

References