Detailed information of TA system TA063325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1281318..1282003
• Replicon: chromosome
• Accession: NZ_CP015736
• Organism: Shinella sp. HZN7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: shn_RS06160
• Protein ID: WP_206362213.1
• Coordinates: 1281605..1282003 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4R2D1Z1
• Locus tag: shn_RS06155
• Protein ID: WP_064329618.1
• Coordinates: 1281318..1281590 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
shn_RS06125	1276953..1277222	-	270	WP_064329612.1	hypothetical protein	-
shn_RS06130	1277315..1277734	-	420	WP_064329613.1	TIGR02301 family protein	-
shn_RS06135	1277731..1278165	-	435	WP_064329614.1	NUDIX hydrolase	-
shn_RS06140	1278226..1279005	+	780	WP_064329615.1	SOS response-associated peptidase	-
shn_RS06145	1279037..1279216	-	180	WP_064329616.1	hypothetical protein	-
shn_RS06150	1279821..1281254	+	1434	WP_064329617.1	cysteine--tRNA ligase	-
shn_RS06155	1281318..1281590	+	273	WP_064329618.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
shn_RS06160	1281605..1282003	+	399	WP_206362213.1	PIN domain-containing protein	Toxin
shn_RS06165	1282000..1282482	+	483	WP_064329620.1	GFA family protein	-
shn_RS06170	1282479..1282976	+	498	WP_064329621.1	GFA family protein	-
shn_RS06175	1283103..1283585	+	483	WP_064329622.1	GFA family protein	-
shn_RS06180	1283582..1284541	+	960	WP_064329623.1	prolyl aminopeptidase	-
shn_RS06185	1284646..1286262	+	1617	WP_064329624.1	citramalate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• SpoVT-AbrB domain (20-58)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14400.50 Da
• Isoelectric Point: 5.2168

>T63325 WP_206362213.1 NZ_CP015736:1281605-1282003 [Shinella sp. HZN7]
MLDTNAISEMVRNPDGTLADRVKAAPTDALCTSAIVVSELRYGLRKRGAVSLARRVEAVLGEIAILPYDTPVSFAYAQTR
DALERRGQPIGYTDLFIAAHALSLELTLVTANIREFSRVEGLKVENWLEAGP

Nucleotide

• Length: 399 bp

>T63325 NZ_CP015736:1281605-1282003 [Shinella sp. HZN7]
ATGCTGGATACGAATGCCATCTCGGAGATGGTCCGCAATCCTGATGGGACGCTCGCTGATCGGGTGAAGGCCGCGCCGAC
GGACGCATTGTGCACGAGCGCCATCGTGGTTTCGGAGCTGCGTTACGGTCTGCGGAAAAGGGGAGCGGTTTCGCTCGCCC
GTCGTGTCGAGGCCGTCCTGGGGGAGATTGCGATCCTCCCCTATGATACGCCCGTTTCCTTCGCTTATGCGCAAACGCGC
GATGCACTGGAGAGAAGGGGCCAGCCGATCGGCTATACCGACCTTTTCATCGCCGCCCATGCTCTCTCGCTGGAACTCAC
GCTCGTCACCGCCAATATCCGTGAATTCTCGCGTGTCGAGGGCCTGAAGGTCGAGAACTGGCTGGAGGCAGGTCCATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10177.60 Da
• Isoelectric Point: 4.4766

>AT63325 WP_064329618.1 NZ_CP015736:1281318-1281590 [Shinella sp. HZN7]
MNARPKEAPDVVQGREVKLFRNGRNQAVRIPVEFELPGNEAVMHKEGDRLIIEPKPKKMGLAALLATFETWEGDFPELDE
TQPPPEDVEL

Nucleotide

• Length: 273 bp

>AT63325 NZ_CP015736:1281318-1281590 [Shinella sp. HZN7]
ATGAATGCAAGACCTAAGGAAGCGCCTGATGTGGTGCAGGGGCGGGAGGTGAAGCTCTTCCGTAACGGCCGCAACCAGGC
CGTCCGGATTCCGGTGGAATTCGAACTGCCGGGAAACGAGGCGGTCATGCACAAGGAAGGTGACCGTCTCATCATCGAGC
CAAAGCCGAAGAAAATGGGGCTCGCCGCGTTGTTGGCGACCTTCGAAACCTGGGAAGGGGACTTTCCGGAACTCGACGAG
ACGCAGCCGCCGCCGGAGGATGTCGAGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A4R2D1Z1