Detailed information of TA system TA063324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 273238..273716
• Replicon: chromosome
• Accession: NZ_CP015736
• Organism: Shinella sp. HZN7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: shn_RS36750
• Protein ID: WP_206362195.1
• Coordinates: 273513..273716 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4R2CE61
• Locus tag: shn_RS01345
• Protein ID: WP_064328833.1
• Coordinates: 273238..273516 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
shn_RS01315	269045..269464	+	420	WP_064328828.1	transcriptional repressor	-
shn_RS01320	269522..270157	-	636	WP_064328829.1	trimeric intracellular cation channel family protein	-
shn_RS01325	270296..270769	+	474	WP_084437695.1	hypothetical protein	-
shn_RS01330	270772..271563	-	792	WP_064328830.1	YdcF family protein	-
shn_RS01335	271595..272767	-	1173	WP_064328831.1	TMEM43 family protein	-
shn_RS01340	272937..273206	+	270	WP_064328832.1	DUF2934 domain-containing protein	-
shn_RS01345	273238..273516	-	279	WP_064328833.1	helix-turn-helix domain-containing protein	Antitoxin
shn_RS36750	273513..273716	-	204	WP_206362195.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
shn_RS01360	274202..274591	-	390	WP_064328834.1	TIGR02300 family protein	-
shn_RS01365	274863..276218	+	1356	WP_064328835.1	3-phosphoshikimate 1-carboxyvinyltransferase	-
shn_RS36265	276222..276365	+	144	WP_162853145.1	hypothetical protein	-
shn_RS01370	276380..277009	+	630	WP_064328836.1	(d)CMP kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (3-63)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-86)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 7632.20 Da
• Isoelectric Point: 11.7182

>T63324 WP_206362195.1 NZ_CP015736:c273716-273513 [Shinella sp. HZN7]
MRGPHVKHLEGKLWELRAKAFEGIARGIYVTVTGRRVVILHVFVKKSQKTPKAALEIARARMKEVKP

Nucleotide

• Length: 204 bp

>T63324 NZ_CP015736:c273716-273513 [Shinella sp. HZN7]
TTGCGGGGACCGCACGTCAAGCACCTAGAAGGCAAGCTTTGGGAACTGCGCGCCAAGGCTTTCGAAGGTATCGCACGCGG
CATTTACGTGACCGTCACCGGACGGCGCGTGGTCATCCTGCATGTCTTCGTGAAGAAGTCGCAGAAGACCCCGAAAGCCG
CCCTTGAGATTGCGAGAGCGCGAATGAAAGAGGTGAAGCCATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10214.78 Da
• Isoelectric Point: 10.2562

>AT63324 WP_064328833.1 NZ_CP015736:c273516-273238 [Shinella sp. HZN7]
MTKIADLKKKLMDNPEFRQEYEKADAEFRLIETLVHARTKAKLSQAELAKRIGTTQSAIARLEGGGVSPSLSTLRRYAEA
TGARLEINLVTP

Nucleotide

• Length: 279 bp

>AT63324 NZ_CP015736:c273516-273238 [Shinella sp. HZN7]
ATGACGAAGATTGCAGACCTCAAGAAGAAGCTGATGGACAACCCGGAATTCCGGCAGGAATACGAGAAGGCCGACGCGGA
ATTCCGGCTTATCGAAACCCTCGTCCATGCCAGGACGAAGGCCAAGCTTTCCCAGGCCGAACTCGCAAAGCGCATCGGCA
CGACGCAATCGGCCATCGCTCGATTGGAAGGGGGCGGCGTTTCCCCTTCCCTCTCTACCCTGCGCCGATATGCCGAGGCC
ACTGGAGCCAGGCTCGAAATCAATCTGGTGACGCCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A4R2CE61