Detailed information of TA system TA063010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 135811..136454
• Replicon: plasmid pNY9_2
• Accession: NZ_CP015387
• Organism: Klebsiella pneumoniae strain NY9

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: A6P56_RS30285
• Protein ID: WP_001044770.1
• Coordinates: 135811..136227 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: A6P56_RS30290
• Protein ID: WP_001261282.1
• Coordinates: 136224..136454 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6P56_RS34190	131702..131767	+	66	Protein_153	EAL domain-containing protein	-
A6P56_RS30245	131907..132671	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
A6P56_RS30260	132814..133080	-	267	WP_001144737.1	plasmid mobilization relaxosome protein MobC	-
A6P56_RS30265	133301..133774	-	474	WP_004201280.1	trimethoprim-resistant dihydrofolate reductase DfrA14	-
A6P56_RS30270	133930..134697	+	768	Protein_157	class 1 integron integrase IntI1	-
A6P56_RS30275	134750..135454	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
A6P56_RS30280	135501..135737	-	237	WP_001348075.1	AAA family ATPase	-
A6P56_RS30285	135811..136227	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
A6P56_RS30290	136224..136454	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A6P56_RS30295	136411..136871	+	461	Protein_162	hypothetical protein	-
A6P56_RS34195	136999..137319	+	321	WP_032072060.1	hypothetical protein	-
A6P56_RS30300	137368..138006	+	639	WP_022644887.1	hypothetical protein	-
A6P56_RS30305	138009..138794	+	786	WP_022644886.1	site-specific integrase	-
A6P56_RS30310	138852..139109	-	258	WP_032408936.1	hypothetical protein	-
A6P56_RS34200	139238..139351	-	114	WP_032072094.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14	-	1..139933	139933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T63010 WP_001044770.1 NZ_CP015387:c136227-135811 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T63010 NZ_CP015387:c136227-135811 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT63010 WP_001261282.1 NZ_CP015387:c136454-136224 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT63010 NZ_CP015387:c136454-136224 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3