Detailed information of TA system TA062937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 723791..724377
• Replicon: chromosome
• Accession: NZ_CP015377
• Organism: Pseudomonas aeruginosa strain BAMCPA07-48

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: A6R75_RS03475
• Protein ID: WP_003120987.1
• Coordinates: 724078..724377 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: A6R75_RS03470
• Protein ID: WP_003448662.1
• Coordinates: 723791..724081 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6R75_RS03450	718942..719151	+	210	WP_003105733.1	cold-shock protein	-
A6R75_RS03455	719373..721262	+	1890	WP_063456059.1	hypothetical protein	-
A6R75_RS03460	721259..723235	+	1977	WP_023980483.1	DEAD/DEAH box helicase	-
A6R75_RS03465	723376..723720	+	345	WP_003448665.1	hypothetical protein	-
A6R75_RS03470	723791..724081	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
A6R75_RS03475	724078..724377	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6R75_RS03480	724579..725703	+	1125	WP_023980489.1	TcpQ domain-containing protein	-
A6R75_RS03485	725703..727412	+	1710	WP_063456060.1	PilN family type IVB pilus formation outer membrane protein	-
A6R75_RS03490	727416..728741	+	1326	WP_063456061.1	type 4b pilus protein PilO2	-
A6R75_RS03495	728731..729264	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T62937 WP_003120987.1 NZ_CP015377:c724377-724078 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T62937 NZ_CP015377:c724377-724078 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATTCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT62937 WP_003448662.1 NZ_CP015377:c724081-723791 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT62937 NZ_CP015377:c724081-723791 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAGTCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTAA

Structures

Toxin

• AlphaFold DB: G8CP73