Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 54691..55241 Replicon chromosome
Accession NZ_CP015229
Organism Escherichia coli strain 06-00048

Toxin (Protein)


Gene name ccdB Uniprot ID A0A193LLV1
Locus tag GJ11_RS02195 Protein ID WP_065226069.1
Coordinates 54927..55241 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag GJ11_RS02190 Protein ID WP_000125568.1
Coordinates 54691..54924 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ11_RS02165 49765..50052 + 288 WP_000203741.1 ferredoxin-like protein FixX -
GJ11_RS02170 50110..51441 + 1332 WP_065226068.1 MFS transporter -
GJ11_RS02175 51549..52079 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
GJ11_RS02180 52072..53934 + 1863 WP_000377114.1 glutathione-regulated potassium-efflux system protein KefC -
GJ11_RS02185 54126..54605 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
GJ11_RS02190 54691..54924 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
GJ11_RS02195 54927..55241 + 315 WP_065226069.1 CcdB family protein Toxin
GJ11_RS02200 55238..56086 - 849 WP_000257196.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
GJ11_RS02205 56093..56470 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
GJ11_RS02210 56473..57294 - 822 WP_001065361.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
GJ11_RS02215 57291..58280 - 990 WP_065226070.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
GJ11_RS02220 58280..59566 - 1287 WP_065226071.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11684.65 Da        Isoelectric Point: 8.0423

>T62668 WP_065226069.1 NZ_CP015229:54927-55241 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TTFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T62668 NZ_CP015229:54927-55241 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGACATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT62668 WP_000125568.1 NZ_CP015229:54691-54924 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT62668 NZ_CP015229:54691-54924 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193LLV1

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References