Detailed information of TA system TA062562

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20582..20846
• Replicon: plasmid pECO-93a
• Accession: NZ_CP015161
• Organism: Escherichia coli strain Eco889

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: WM90_RS26320
• Protein ID: WP_001331364.1
• Coordinates: 20582..20734 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	20789..20846 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM90_RS26280	16934..17596	+	663	WP_064847761.1	plasmid IncI1-type surface exclusion protein ExcA	-
WM90_RS26285	17694..17903	+	210	WP_001140543.1	hemolysin expression modulator Hha	-
WM90_RS26295	18147..18809	+	663	WP_042078443.1	plasmid IncI1-type surface exclusion protein ExcA	-
WM90_RS26300	18881..19090	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
WM90_RS30730	19422..19598	+	177	WP_001054900.1	hypothetical protein	-
WM90_RS26310	19663..19959	-	297	WP_001275298.1	hypothetical protein	-
WM90_RS26315	20259..20510	+	252	WP_001291965.1	hypothetical protein	-
WM90_RS26320	20582..20734	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	20789..20846	+	58	NuclAT_0	-	Antitoxin
-	20789..20846	+	58	NuclAT_0	-	Antitoxin
-	20789..20846	+	58	NuclAT_0	-	Antitoxin
-	20789..20846	+	58	NuclAT_0	-	Antitoxin
WM90_RS26325	21026..22234	+	1209	WP_064847762.1	IncI1-type conjugal transfer protein TrbA	-
WM90_RS26330	22253..23323	+	1071	WP_064847763.1	IncI1-type conjugal transfer protein TrbB	-
WM90_RS26335	23316..25607	+	2292	WP_077883636.1	conjugal transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88023	88023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T62562 WP_001331364.1 NZ_CP015161:c20734-20582 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T62562 NZ_CP015161:c20734-20582 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT62562 NZ_CP015161:20789-20846 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin