Detailed information of TA system TA062272

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 99734..99973
• Replicon: plasmid pEC448_1
• Accession: NZ_CP015077
• Organism: Escherichia coli strain Ecol_448

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: A4R37_RS25540
• Protein ID: WP_023144756.1
• Coordinates: 99734..99868 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	99913..99973 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R37_RS25510	95400..97013	-	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
A4R37_RS25515	97044..97394	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
A4R37_RS25520	97391..97816	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
A4R37_RS25525	98022..98879	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
A4R37_RS25530	98872..98946	-	75	WP_031943482.1	RepA leader peptide Tap	-
A4R37_RS29825	98943..99077	-	135	Protein_126	protein CopA/IncA	-
A4R37_RS25535	99183..99437	-	255	WP_000083850.1	replication regulatory protein RepA	-
A4R37_RS25540	99734..99868	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	99913..99973	+	61	NuclAT_2	-	Antitoxin
-	99913..99973	+	61	NuclAT_2	-	Antitoxin
-	99913..99973	+	61	NuclAT_2	-	Antitoxin
-	99913..99973	+	61	NuclAT_2	-	Antitoxin
A4R37_RS25545	99940..100226	-	287	Protein_129	DUF2726 domain-containing protein	-
A4R37_RS25550	100739..100951	-	213	WP_013023861.1	hypothetical protein	-
A4R37_RS25555	101082..101642	-	561	WP_000139341.1	fertility inhibition protein FinO	-
A4R37_RS25560	101697..102443	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..133735	133735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T62272 WP_023144756.1 NZ_CP015077:c99868-99734 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T62272 NZ_CP015077:c99868-99734 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT62272 NZ_CP015077:99913-99973 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin