Detailed information of TA system TA061973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5034891..5035572
• Replicon: chromosome
• Accession: NZ_CP014999
• Organism: Pseudomonas aeruginosa strain PA7790

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PA7790_RS23560
• Protein ID: WP_015503432.1
• Coordinates: 5035207..5035572 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA7790_RS23555
• Protein ID: WP_016561576.1
• Coordinates: 5034891..5035214 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA7790_RS23530	5030512..5031390	+	879	WP_003109435.1	hypothetical protein	-
PA7790_RS34680	5031902..5032768	-	867	WP_023081971.1	hypothetical protein	-
PA7790_RS34685	5032798..5033049	-	252	WP_124136459.1	hypothetical protein	-
PA7790_RS34125	5033266..5033820	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
PA7790_RS34130	5033994..5034269	-	276	WP_004353146.1	hypothetical protein	-
PA7790_RS23545	5034269..5034601	-	333	WP_016561578.1	hypothetical protein	-
PA7790_RS34690	5034656..5034775	+	120	Protein_4703	IS5/IS1182 family transposase	-
PA7790_RS23555	5034891..5035214	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
PA7790_RS23560	5035207..5035572	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA7790_RS23565	5035836..5036078	-	243	WP_043884955.1	hypothetical protein	-
PA7790_RS23570	5036285..5036557	+	273	WP_003085667.1	hypothetical protein	-
PA7790_RS23575	5036576..5037001	-	426	WP_003163196.1	VOC family protein	-
PA7790_RS23580	5037102..5037986	+	885	WP_019725847.1	LysR family transcriptional regulator	-
PA7790_RS23585	5037959..5038912	-	954	WP_003085661.1	LysR family transcriptional regulator	-
PA7790_RS23590	5039133..5039567	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T61973 WP_015503432.1 NZ_CP014999:c5035572-5035207 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T61973 NZ_CP014999:c5035572-5035207 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT61973 WP_016561576.1 NZ_CP014999:c5035214-5034891 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT61973 NZ_CP014999:c5035214-5034891 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA