Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 31825..32426 Replicon plasmid pL11994-1
Accession NZ_CP014916
Organism Secundilactobacillus paracollinoides strain TMW 1.1994

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag AYR62_RS15635 Protein ID WP_001748110.1
Coordinates 31825..32169 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag AYR62_RS15640 Protein ID WP_001748109.1
Coordinates 32163..32426 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR62_RS15620 26923..27723 - 801 WP_065928768.1 AAA family ATPase -
AYR62_RS15625 28925..30547 + 1623 WP_065928769.1 type I restriction-modification system subunit M -
AYR62_RS15630 30537..31715 + 1179 WP_065928770.1 restriction endonuclease subunit S -
AYR62_RS15635 31825..32169 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AYR62_RS15640 32163..32426 - 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYR62_RS15645 32512..33099 + 588 WP_041153703.1 site-specific integrase -
AYR62_RS15650 33552..33851 + 300 Protein_36 helix-turn-helix domain-containing protein -
AYR62_RS15655 34341..34799 + 459 WP_065928771.1 phage terminase small subunit P27 family -
AYR62_RS15660 34952..35371 - 420 WP_065928772.1 hypothetical protein -
AYR62_RS15665 35671..36015 - 345 WP_156771286.1 hypothetical protein -
AYR62_RS15670 36387..37421 + 1035 WP_057910131.1 putrescine carbamoyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..45958 45958


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T61890 WP_001748110.1 NZ_CP014916:c32169-31825 [Secundilactobacillus paracollinoides]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T61890 NZ_CP014916:c32169-31825 [Secundilactobacillus paracollinoides]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTGGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT61890 WP_001748109.1 NZ_CP014916:c32426-32163 [Secundilactobacillus paracollinoides]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT61890 NZ_CP014916:c32426-32163 [Secundilactobacillus paracollinoides]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAGCTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References