Detailed information of TA system TA061865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2642646..2643259
• Replicon: chromosome
• Accession: NZ_CP014867
• Organism: Pseudomonas chlororaphis strain 189 isolate 189

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A285EYZ4
• Locus tag: A3218_RS11420
• Protein ID: WP_009041685.1
• Coordinates: 2643077..2643259 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A3218_RS11415
• Protein ID: WP_062826224.1
• Coordinates: 2642646..2643047 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3218_RS11400	2637674..2638999	-	1326	WP_062824002.1	LLM class flavin-dependent oxidoreductase	-
A3218_RS11405	2639046..2641304	-	2259	WP_062824003.1	TonB-dependent receptor	-
A3218_RS11410	2641511..2642557	+	1047	WP_062824004.1	LacI family DNA-binding transcriptional regulator	-
A3218_RS11415	2642646..2643047	-	402	WP_062826224.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A3218_RS11420	2643077..2643259	-	183	WP_009041685.1	type II toxin-antitoxin system HicA family toxin	Toxin
A3218_RS11425	2643384..2644274	-	891	WP_053259258.1	LysR family transcriptional regulator	-
A3218_RS11430	2644413..2645153	+	741	WP_062824005.1	SDR family oxidoreductase	-
A3218_RS11435	2645187..2645696	+	510	WP_062824006.1	DUF1097 domain-containing protein	-
A3218_RS11440	2645726..2646622	+	897	WP_062824007.1	SDR family oxidoreductase	-
A3218_RS11445	2646696..2647184	+	489	WP_062824008.1	DoxX family protein	-
A3218_RS11450	2647237..2647494	+	258	WP_062824009.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.08 Da
• Isoelectric Point: 10.4588

>T61865 WP_009041685.1 NZ_CP014867:c2643259-2643077 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T61865 NZ_CP014867:c2643259-2643077 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAGCATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14628.50 Da
• Isoelectric Point: 4.9133

>AT61865 WP_062826224.1 NZ_CP014867:c2643047-2642646 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAEAFENVKEALSLHYEGLVADGQPLPQVHEVDVHIDNPDYAGGVWGMI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT61865 NZ_CP014867:c2643047-2642646 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCGGCAGAAGCCTTCGAGAATGTGAAAGAGGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTCAACCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGCATGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGCTTCAACGCCACCCTGCCGGAGCAATTGCTGGAACGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGCTCGCGTTCGGGCTTCCTTGCCGCGGCGGCATTGCGCGAGTTATCGGCGT
AG

Structures

Toxin

• AlphaFold DB: A0A285EYZ4