Detailed information of TA system TA061860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 553995..554650
• Replicon: chromosome
• Accession: NZ_CP014867
• Organism: Pseudomonas chlororaphis strain 189 isolate 189

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7D6EBU8
• Locus tag: A3218_RS02150
• Protein ID: WP_062823026.1
• Coordinates: 554306..554650 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A3218_RS02145
• Protein ID: WP_062823025.1
• Coordinates: 553995..554309 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3218_RS02105	549971..550777	-	807	WP_062823017.1	ATP-binding protein	-
A3218_RS32010	550758..551534	-	777	WP_062823018.1	hypothetical protein	-
A3218_RS02115	551538..551759	-	222	WP_062823019.1	hypothetical protein	-
A3218_RS02120	551756..552019	-	264	WP_062823020.1	hypothetical protein	-
A3218_RS02125	552016..552399	-	384	WP_155736123.1	hypothetical protein	-
A3218_RS02130	552625..553047	-	423	WP_062823022.1	Rha family transcriptional regulator	-
A3218_RS02135	553172..553417	-	246	WP_062823023.1	helix-turn-helix domain-containing protein	-
A3218_RS02140	553523..553942	+	420	WP_202908057.1	hypothetical protein	-
A3218_RS02145	553995..554309	-	315	WP_062823025.1	transcriptional regulator	Antitoxin
A3218_RS02150	554306..554650	-	345	WP_062823026.1	hypothetical protein	Toxin
A3218_RS02155	554784..555587	-	804	WP_062823027.1	phage antirepressor KilAC domain-containing protein	-
A3218_RS02160	555587..555895	-	309	WP_062823028.1	DUF3077 domain-containing protein	-
A3218_RS30440	555960..556142	-	183	WP_162270002.1	Arc family DNA-binding protein	-
A3218_RS30445	556261..556674	+	414	WP_081232211.1	Arc family DNA-binding protein	-
A3218_RS02165	556873..557790	-	918	WP_155736124.1	hypothetical protein	-
A3218_RS02175	558462..559115	-	654	WP_155736125.1	hypothetical protein	-
A3218_RS02180	559152..559361	-	210	WP_062823032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	501898..562811	60913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13208.96 Da
• Isoelectric Point: 9.6391

>T61860 WP_062823026.1 NZ_CP014867:c554650-554306 [Pseudomonas chlororaphis]
MKTVFFETTSFTATVGDYLTDDEYRQLQAEMQANPTAGDVMPRTGGFRKLRWHDSRRGKGKRGGLRVIYYWLLDNDQFWM
FSIYDKDEVTKLTSDQEKALKAAIDAELKKRGGK

Nucleotide

• Length: 345 bp

>T61860 NZ_CP014867:c554650-554306 [Pseudomonas chlororaphis]
ATGAAAACAGTCTTCTTTGAAACTACAAGCTTTACCGCCACGGTAGGCGACTATCTGACTGACGATGAATATCGGCAGCT
TCAAGCGGAGATGCAGGCGAACCCTACGGCTGGCGATGTGATGCCGCGTACGGGGGGATTCAGAAAGCTTCGTTGGCATG
ACTCCCGTAGAGGAAAGGGGAAGCGTGGCGGGCTTCGTGTCATCTACTACTGGCTGCTTGATAACGATCAGTTTTGGATG
TTTTCGATCTACGACAAGGACGAGGTTACAAAGCTGACTTCCGACCAGGAGAAGGCACTGAAAGCTGCGATTGATGCAGA
GCTGAAAAAGCGAGGTGGGAAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12018.03 Da
• Isoelectric Point: 10.0926

>AT61860 WP_062823025.1 NZ_CP014867:c554309-553995 [Pseudomonas chlororaphis]
MKKRDLFAELMQGVEEMGEHRKGKITLRQYEMEALPAPEVTAAEIISIREKLHMSQPVFARQIRTSPDTLKNWEQSKSKP
NAQAALLIKLVQRFPDMVERLNTV

Nucleotide

• Length: 315 bp

>AT61860 NZ_CP014867:c554309-553995 [Pseudomonas chlororaphis]
ATGAAAAAGCGCGATCTATTTGCTGAGTTGATGCAAGGTGTGGAAGAGATGGGCGAGCATCGGAAGGGGAAGATCACTCT
TCGTCAGTACGAGATGGAAGCTCTCCCTGCACCAGAGGTGACTGCCGCTGAGATCATTTCTATCCGTGAAAAGCTTCATA
TGTCGCAGCCAGTGTTCGCCAGACAAATTCGCACCAGTCCGGACACGCTGAAAAATTGGGAGCAGTCGAAGTCAAAGCCG
AATGCCCAGGCAGCATTGCTGATTAAGCTGGTTCAGCGTTTCCCTGATATGGTCGAGCGTCTTAACACTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A7D6EBU8