Detailed information of TA system TA061789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 209408..210318
• Replicon: plasmid pKPN-332
• Accession: NZ_CP014763
• Organism: Klebsiella pneumoniae strain KPNIH39

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: WM47_RS27815
• Protein ID: WP_040217285.1
• Coordinates: 209848..210318 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: WM47_RS27810
• Protein ID: WP_032413443.1
• Coordinates: 209408..209851 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM47_RS27790	205692..207110	+	1419	WP_040217041.1	UvrD-helicase domain-containing protein	-
WM47_RS27795	207170..207496	+	327	WP_142390228.1	hypothetical protein	-
WM47_RS27800	207522..208490	-	969	WP_016151349.1	IS5 family transposase	-
WM47_RS30520	208510..208695	+	186	WP_071953846.1	DUF2188 domain-containing protein	-
WM47_RS27805	208796..209308	+	513	Protein_209	DUF4113 domain-containing protein	-
WM47_RS27810	209408..209851	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
WM47_RS27815	209848..210318	+	471	WP_040217285.1	RES family NAD+ phosphorylase	Toxin
WM47_RS27820	210428..210688	-	261	WP_017901409.1	hypothetical protein	-
WM47_RS27825	211260..212303	-	1044	Protein_213	DDE-type integrase/transposase/recombinase	-
WM47_RS27830	212339..213307	-	969	WP_077268204.1	IS5 family transposase	-
WM47_RS27835	213737..214921	+	1185	WP_040217080.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / pla	1..284894	284894
	-	inside	IScluster/Tn	-	-	207522..213307	5785

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17601.88 Da
• Isoelectric Point: 4.5152

>T61789 WP_040217285.1 NZ_CP014763:209848-210318 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAVQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T61789 NZ_CP014763:209848-210318 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGTACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT61789 WP_032413443.1 NZ_CP014763:209408-209851 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT61789 NZ_CP014763:209408-209851 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4