Detailed information of TA system TA061536

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3628673..3629510
• Replicon: chromosome
• Accession: NZ_CP014583
• Organism: Escherichia coli strain CFSAN004176

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: BCV59_RS19565
• Protein ID: WP_000854687.1
• Coordinates: 3628673..3629053 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LJJ3
• Locus tag: BCV59_RS19570
• Protein ID: WP_001443392.1
• Coordinates: 3629142..3629510 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV59_RS19535	3624316..3626157	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
BCV59_RS19540	3626235..3626741	-	507	WP_069357448.1	G/U mismatch-specific DNA glycosylase	-
BCV59_RS19550	3627041..3627886	-	846	WP_001274557.1	DUF4942 domain-containing protein	-
BCV59_RS19555	3627971..3628168	-	198	WP_000772026.1	DUF957 domain-containing protein	-
BCV59_RS19560	3628188..3628676	-	489	WP_001054228.1	hypothetical protein	-
BCV59_RS19565	3628673..3629053	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
BCV59_RS19570	3629142..3629510	-	369	WP_001443392.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BCV59_RS19575	3629586..3629807	-	222	WP_000692312.1	DUF987 domain-containing protein	-
BCV59_RS19580	3629870..3630346	-	477	WP_001186728.1	RadC family protein	-
BCV59_RS19585	3630362..3630835	-	474	WP_001444656.1	antirestriction protein	-
BCV59_RS19590	3630929..3631174	-	246	WP_000680587.1	hypothetical protein	-
BCV59_RS19595	3631174..3631614	-	441	Protein_3492	DUF932 domain-containing protein	-
BCV59_RS19600	3631733..3633511	-	1779	WP_001243195.1	reverse transcriptase/maturase family protein	-
BCV59_RS19605	3634021..3634404	-	384	Protein_3494	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	3586096..3636933	50837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T61536 WP_000854687.1 NZ_CP014583:c3629053-3628673 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T61536 NZ_CP014583:c3629053-3628673 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13781.54 Da
• Isoelectric Point: 6.2049

>AT61536 WP_001443392.1 NZ_CP014583:c3629510-3629142 [Escherichia coli]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT61536 NZ_CP014583:c3629510-3629142 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTAGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67

Antitoxin

• AlphaFold DB: A0A7U9LJJ3