Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-PHD
Location 3783361..3784061 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name vapC Uniprot ID O50411
Locus tag AZH48_RS17700 Protein ID WP_003417916.1
Coordinates 3783361..3783753 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WF13
Locus tag AZH48_RS17705 Protein ID WP_003417918.1
Coordinates 3783753..3784061 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS17685 3778731..3780569 - 1839 WP_011799348.1 1-deoxy-D-xylulose-5-phosphate synthase -
AZH48_RS17690 3780566..3781555 - 990 WP_003417912.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
AZH48_RS17695 3781555..3782607 - 1053 WP_003900678.1 polyprenyl synthetase family protein -
AZH48_RS17700 3783361..3783753 - 393 WP_003417916.1 type II toxin-antitoxin system VapC family toxin Toxin
AZH48_RS17705 3783753..3784061 - 309 WP_003417918.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AZH48_RS21455 3784240..3785581 + 1342 Protein_3483 ISNCY family transposase -
AZH48_RS17720 3785771..3788023 + 2253 WP_011799349.1 PE family protein -
AZH48_RS17725 3788094..3788966 - 873 WP_003417929.1 3-hydroxyacyl-thioester dehydratase HtdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3784904..3785581 677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(12-64)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14316.74 Da        Isoelectric Point: 10.7249

>T61497 WP_003417916.1 NZ_CP014566:c3783753-3783361 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Download         Length: 393 bp

>T61497 NZ_CP014566:c3783753-3783361 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11030.59 Da        Isoelectric Point: 4.5332

>AT61497 WP_003417918.1 NZ_CP014566:c3784061-3783753 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MTPTACATVSTMTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEP
LDLDAGVELPSVTLARLREHER

Download         Length: 309 bp

>AT61497 NZ_CP014566:c3784061-3783753 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGACGCCGACCGCTTGTGCTACAGTTAGCACAATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCT
GCTGCGCCGAGTCGAGGCCGGCGAGACGATCGAGATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGC
AGGGCGGTCCATACGAGCAGCTGCTGGCAAGCGGCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCG
CTCGACCTCGACGCGGGCGTCGAGCTGCCGTCGGTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BXS8

Antitoxin

Source ID Structure
PDB 6JQY
AlphaFold DB P9WF13

References