Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/Txe-YefM
Location 3756239..3756768 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name relE Uniprot ID G0TI95
Locus tag AZH48_RS17585 Protein ID WP_003417760.1
Coordinates 3756511..3756768 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G0TI94
Locus tag AZH48_RS17580 Protein ID WP_003417757.1
Coordinates 3756239..3756514 (+) Length 92 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS17545 3751270..3752568 - 1299 WP_011799343.1 PPE family protein -
AZH48_RS17550 3752812..3753606 - 795 WP_003417738.1 BBE domain-containing protein -
AZH48_RS17555 3753688..3754059 - 372 WP_003417739.1 FAD-binding oxidoreductase -
AZH48_RS22345 3753957..3754175 - 219 WP_157132559.1 hypothetical protein -
AZH48_RS17560 3754202..3754462 - 261 WP_003417741.1 hypothetical protein -
AZH48_RS17565 3754577..3754966 + 390 WP_010950876.1 DUF732 domain-containing protein -
AZH48_RS17570 3754980..3755273 - 294 WP_003417749.1 DUF3017 domain-containing protein -
AZH48_RS17575 3755270..3756115 - 846 WP_010950877.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase -
AZH48_RS17580 3756239..3756514 + 276 WP_003417757.1 type II toxin-antitoxin system antitoxin RelJ Antitoxin
AZH48_RS17585 3756511..3756768 + 258 WP_003417760.1 Txe/YoeB family addiction module toxin Toxin
AZH48_RS17590 3756810..3758000 + 1191 WP_003900033.1 NADH:flavin oxidoreductase -
AZH48_RS17595 3758117..3758485 + 369 WP_003417765.1 FHA domain-containing protein -
AZH48_RS17600 3758482..3759033 - 552 WP_003417767.1 pentapeptide repeat protein MfpA -
AZH48_RS17605 3759040..3759621 - 582 WP_003417769.1 ATP/GTP-binding protein -
AZH48_RS17610 3759602..3759970 - 369 WP_003417772.1 DUF742 domain-containing protein -
AZH48_RS17615 3759948..3760340 - 393 WP_011799344.1 roadblock/LC7 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-85)

Antitoxin

(2-72)


Sequences


Toxin        


Download         Length: 86 a.a.        Molecular weight: 10070.30 Da        Isoelectric Point: 6.4693

>T61496 WP_003417760.1 NZ_CP014566:3756511-3756768 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Download         Length: 258 bp

>T61496 NZ_CP014566:3756511-3756768 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10194.35 Da        Isoelectric Point: 4.6652

>AT61496 WP_003417757.1 NZ_CP014566:3756239-3756514 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Download         Length: 276 bp

>AT61496 NZ_CP014566:3756239-3756514 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3OEI


Antitoxin

Source ID Structure
PDB 3CTO
PDB 3OEI
PDB 3D55
AlphaFold DB G0TI94

References