Detailed information of TA system TA061480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2374974..2375503
• Replicon: chromosome
• Accession: NZ_CP014566
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: AZH48_RS11070
• Protein ID: WP_003411124.1
• Coordinates: 2374974..2375291 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: AZH48_RS11075
• Protein ID: WP_003411127.1
• Coordinates: 2375288..2375503 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZH48_RS11040	2370111..2371187	+	1077	WP_003411110.1	hypothetical protein	-
AZH48_RS11045	2371184..2371465	+	282	WP_019283661.1	hypothetical protein	-
AZH48_RS11050	2371501..2372574	+	1074	WP_011799246.1	quinone-dependent dihydroorotate dehydrogenase	-
AZH48_RS11055	2372579..2373109	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
AZH48_RS11060	2373157..2374503	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
AZH48_RS11070	2374974..2375291	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AZH48_RS11075	2375288..2375503	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
AZH48_RS11080	2375758..2376816	+	1059	WP_003411129.1	hypothetical protein	-
AZH48_RS11085	2376946..2377302	-	357	WP_003411130.1	hypothetical protein	-
AZH48_RS11090	2377397..2378179	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
AZH48_RS11095	2378447..2378737	-	291	WP_003900476.1	YggT family protein	-
AZH48_RS11100	2378899..2379555	-	657	WP_003411133.1	cell division protein SepF	-
AZH48_RS11105	2379621..2380397	-	777	WP_003411137.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T61480 WP_003411124.1 NZ_CP014566:c2375291-2374974 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T61480 NZ_CP014566:c2375291-2374974 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGACGCGTAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT61480 WP_003411127.1 NZ_CP014566:c2375503-2375288 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT61480 NZ_CP014566:c2375503-2375288 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4