Detailed information of TA system TA061476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2229476..2230102
• Replicon: chromosome
• Accession: NZ_CP014566
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: AZH48_RS10375
• Protein ID: WP_003410075.1
• Coordinates: 2229704..2230102 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AZH48_RS10370
• Protein ID: WP_019283586.1
• Coordinates: 2229476..2229703 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZH48_RS10360	2227515..2227859	-	345	WP_003410065.1	ferredoxin family protein	-
AZH48_RS10365	2228048..2229373	-	1326	WP_003410070.1	DUF4143 domain-containing protein	-
AZH48_RS10370	2229476..2229703	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AZH48_RS10375	2229704..2230102	+	399	WP_003410075.1	PIN domain nuclease	Toxin
AZH48_RS10380	2230285..2230716	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
AZH48_RS10385	2230757..2231251	+	495	WP_003410080.1	DUF1398 domain-containing protein	-
AZH48_RS20995	2231688..2231867	-	180	Protein_2038	hypothetical protein	-
AZH48_RS10390	2231928..2232572	+	645	WP_078396697.1	transposase	-
AZH48_RS10395	2232526..2233116	+	591	WP_003899131.1	IS110 family transposase	-
AZH48_RS10400	2233244..2234500	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T61476 WP_003410075.1 NZ_CP014566:2229704-2230102 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T61476 NZ_CP014566:2229704-2230102 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT61476 WP_019283586.1 NZ_CP014566:2229476-2229703 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT61476 NZ_CP014566:2229476-2229703 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG