Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 843090..843799 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4F979
Locus tag AZH48_RS03945 Protein ID WP_003903160.1
Coordinates 843371..843799 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TEX4
Locus tag AZH48_RS03940 Protein ID WP_003403834.1
Coordinates 843090..843347 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS03935 840318..842999 + 2682 WP_061512247.1 PE family protein -
AZH48_RS03940 843090..843347 + 258 WP_003403834.1 ribbon-helix-helix protein, CopG family Antitoxin
AZH48_RS03945 843371..843799 + 429 WP_003903160.1 type II toxin-antitoxin system VapC family toxin Toxin
AZH48_RS03950 843880..844017 - 138 WP_003403839.1 hypothetical protein -
AZH48_RS03955 844176..844421 + 246 WP_003403841.1 hypothetical protein -
AZH48_RS03960 844490..845374 - 885 WP_011799127.1 3-hydroxyisobutyrate dehydrogenase -
AZH48_RS03965 845385..846557 - 1173 WP_010950438.1 acyl-CoA dehydrogenase family protein -
AZH48_RS03970 846564..848096 - 1533 WP_003403847.1 CoA-acylating methylmalonate-semialdehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-133)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-36)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15799.21 Da        Isoelectric Point: 7.5536

>T61457 WP_003903160.1 NZ_CP014566:843371-843799 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLVLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Download         Length: 429 bp

>T61457 NZ_CP014566:843371-843799 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTGGTGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9114.28 Da        Isoelectric Point: 10.6530

>AT61457 WP_003403834.1 NZ_CP014566:843090-843347 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Download         Length: 258 bp

>AT61457 NZ_CP014566:843090-843347 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F979

Antitoxin

Source ID Structure
AlphaFold DB G0TEX4

References