Detailed information of TA system TA061448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 698400..699046
• Replicon: chromosome
• Accession: NZ_CP014566
• Organism: Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: AZH48_RS03125
• Protein ID: WP_003403137.1
• Coordinates: 698400..698813 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: AZH48_RS03130
• Protein ID: WP_003403139.1
• Coordinates: 698810..699046 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZH48_RS03105	694483..696033	+	1551	WP_003403119.1	MCE family protein	-
AZH48_RS03110	696085..696477	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
AZH48_RS03115	696474..696731	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
AZH48_RS03120	696914..698149	-	1236	WP_003403128.1	ATP-binding protein	-
AZH48_RS03125	698400..698813	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
AZH48_RS03130	698810..699046	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AZH48_RS03135	699150..699656	-	507	WP_003900973.1	two component system sensor kinase HK1	-
AZH48_RS03140	699770..700240	-	471	WP_003403144.1	HAMP domain-containing protein	-
AZH48_RS03145	700284..701045	-	762	WP_010950420.1	two-component system response regulator TcrA	-
AZH48_RS03150	701102..701413	+	312	WP_003403164.1	hypothetical protein	-
AZH48_RS03155	701485..702435	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
AZH48_RS03160	702676..703260	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
AZH48_RS03165	703262..703972	+	711	Protein_627	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T61448 WP_003403137.1 NZ_CP014566:c698813-698400 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T61448 NZ_CP014566:c698813-698400 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT61448 WP_003403139.1 NZ_CP014566:c699046-698810 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT61448 NZ_CP014566:c699046-698810 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89