Detailed information of TA system TA061437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 4104695..4105308
• Replicon: chromosome
• Accession: NZ_CP014546
• Organism: Pseudomonas azotoformans strain S4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W2DZB1
• Locus tag: AYR47_RS19200
• Protein ID: WP_033897428.1
• Coordinates: 4105057..4105308 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: W2DXJ4
• Locus tag: AYR47_RS19195
• Protein ID: WP_033897427.1
• Coordinates: 4104695..4105060 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR47_RS19175	4100856..4101428	+	573	WP_051421959.1	DUF2239 family protein	-
AYR47_RS19180	4101454..4102809	+	1356	WP_033897424.1	MATE family efflux transporter	-
AYR47_RS19185	4102793..4103380	-	588	WP_033897425.1	TetR/AcrR family transcriptional regulator	-
AYR47_RS19190	4103522..4104694	+	1173	WP_033897426.1	MFS transporter	-
AYR47_RS19195	4104695..4105060	-	366	WP_033897427.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AYR47_RS19200	4105057..4105308	-	252	WP_033897428.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9561.10 Da
• Isoelectric Point: 10.0941

>T61437 WP_033897428.1 NZ_CP014546:c4105308-4105057 [Pseudomonas azotoformans]
VSKNEQLLAKRLNEHMAFTWPELVTLLRRLGYTQIEGAGSRVKFDIGDPSAMITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T61437 NZ_CP014546:c4105308-4105057 [Pseudomonas azotoformans]
GTGTCCAAAAATGAACAGCTGCTCGCCAAACGGCTCAATGAGCACATGGCATTTACCTGGCCTGAGCTCGTAACGCTGCT
GCGACGACTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGACATCGGCGACCCCAGTGCAATGATCA
CCTTGCACAAGCCTCACCCTGGCAACGAACTGAAACACTACATTCGGCGCCAGATCATCGAACAGTTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13154.95 Da
• Isoelectric Point: 6.9476

>AT61437 WP_033897427.1 NZ_CP014546:c4105060-4104695 [Pseudomonas azotoformans]
VNNQLKHKGYIGSIEASVEDNCLFGKILFIKALVSYEGKTVAELDAAFREAVDDYLATCQTLGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALNEFLQHHGNDACPAVS

Nucleotide

• Length: 366 bp

>AT61437 NZ_CP014546:c4105060-4104695 [Pseudomonas azotoformans]
GTGAACAACCAACTGAAACACAAGGGCTACATCGGCTCCATCGAAGCCAGCGTCGAAGACAACTGCCTGTTCGGCAAGAT
TCTGTTCATCAAGGCGTTGGTGAGCTATGAAGGGAAAACCGTCGCTGAACTGGACGCTGCATTTCGTGAGGCGGTGGATG
ACTACCTCGCCACTTGCCAGACCCTCGGGCAGACCCCGGAGAAGCCCTGCAAGGGTTCCTTCAATGTCCGGGTCGGCCAT
GACTTGCACTTGGCGGCAGCCCTGGCGGCAACCCGCAAAAAAGTGACCCTCAATGACCTGACACGCCAGGCCTTGAATGA
GTTTTTACAGCATCATGGCAATGATGCATGCCCTGCGGTTAGCTGA

Structures

Toxin

• AlphaFold DB: W2DZB1

Antitoxin

• AlphaFold DB: W2DXJ4