Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2785538..2786130 Replicon chromosome
Accession NZ_CP014546
Organism Pseudomonas azotoformans strain S4

Toxin (Protein)


Gene name graT Uniprot ID W2E0K6
Locus tag AYR47_RS12855 Protein ID WP_033896385.1
Coordinates 2785538..2785816 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID W2E2F7
Locus tag AYR47_RS12860 Protein ID WP_033896386.1
Coordinates 2785825..2786130 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR47_RS12835 2781863..2782624 - 762 WP_061435459.1 LuxR family transcriptional regulator -
AYR47_RS12840 2782718..2783539 + 822 WP_061435461.1 carbon-nitrogen hydrolase family protein -
AYR47_RS12845 2783674..2784999 + 1326 WP_033896383.1 APC family permease -
AYR47_RS12850 2785100..2785372 + 273 WP_033896384.1 type B 50S ribosomal protein L31 -
AYR47_RS12855 2785538..2785816 + 279 WP_033896385.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AYR47_RS12860 2785825..2786130 + 306 WP_033896386.1 HigA family addiction module antidote protein Antitoxin
AYR47_RS12865 2786316..2787632 + 1317 Protein_2562 response regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(11-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10560.14 Da        Isoelectric Point: 8.5300

>T61430 WP_033896385.1 NZ_CP014546:2785538-2785816 [Pseudomonas azotoformans]
MIRSFKCVDTKTLFETGRIRRWSAIVSVIERKLSMLDAAIDLADLRSPPGNRLEALYGDREGQHSIRINGQFRICFIWGP
NGPQNVEIVDYH

Download         Length: 279 bp

>T61430 NZ_CP014546:2785538-2785816 [Pseudomonas azotoformans]
ATGATCAGAAGCTTCAAATGCGTGGATACCAAAACCCTTTTTGAAACCGGTCGAATCCGGCGCTGGTCTGCCATCGTGTC
GGTGATTGAACGAAAGCTCAGCATGCTCGATGCGGCCATTGATCTTGCCGACTTGCGCTCGCCACCTGGAAACAGGCTGG
AAGCACTGTACGGTGACAGAGAGGGGCAGCACAGCATCCGAATCAATGGTCAATTCCGGATCTGTTTCATTTGGGGGCCC
AACGGTCCGCAAAACGTTGAAATTGTGGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11011.82 Da        Isoelectric Point: 7.2775

>AT61430 WP_033896386.1 NZ_CP014546:2785825-2786130 [Pseudomonas azotoformans]
MFKNGMRPVHPGEILKEEYLEPLGLTAAALARALHVSAPTVNDIVLMRRSISADVALRLAAALETSAQFWLNLQTAYDLR
TVEIAKGAQISKEVEKVAHCA

Download         Length: 306 bp

>AT61430 NZ_CP014546:2785825-2786130 [Pseudomonas azotoformans]
ATGTTTAAAAACGGCATGCGTCCCGTACACCCTGGAGAGATTCTCAAGGAAGAATACTTGGAGCCGTTGGGCCTTACGGC
TGCAGCCCTCGCGCGGGCGCTGCATGTCTCCGCGCCCACGGTGAATGACATTGTGCTGATGCGCCGTAGCATCAGTGCTG
ATGTGGCGCTTCGCTTGGCCGCTGCGTTGGAAACATCCGCGCAGTTTTGGCTTAACCTGCAGACCGCTTATGATTTGCGT
ACGGTGGAAATTGCCAAGGGCGCTCAGATATCCAAGGAAGTGGAGAAAGTCGCGCATTGCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W2E0K6

Antitoxin

Source ID Structure
AlphaFold DB W2E2F7

References