Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 2205891..2206486 Replicon chromosome
Accession NZ_CP014546
Organism Pseudomonas azotoformans strain S4

Toxin (Protein)


Gene name PumA Uniprot ID A0A127HVR9
Locus tag AYR47_RS10190 Protein ID WP_061435131.1
Coordinates 2206178..2206486 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A127HWA5
Locus tag AYR47_RS10185 Protein ID WP_061435130.1
Coordinates 2205891..2206181 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR47_RS10165 2202315..2204375 - 2061 WP_061435126.1 TonB-dependent copper receptor -
AYR47_RS10170 2204469..2204858 - 390 WP_061435128.1 DUF2946 domain-containing protein -
AYR47_RS10175 2204870..2205353 - 484 Protein_2038 copper chaperone PCu(A)C -
AYR47_RS10180 2205401..2205796 - 396 WP_033897357.1 DUF2946 domain-containing protein -
AYR47_RS10185 2205891..2206181 - 291 WP_061435130.1 putative addiction module antidote protein Antitoxin
AYR47_RS10190 2206178..2206486 - 309 WP_061435131.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AYR47_RS10195 2206844..2207563 - 720 WP_061435133.1 cobalt-precorrin-6A reductase -
AYR47_RS10200 2207560..2208765 - 1206 WP_061435135.1 bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase -
AYR47_RS10205 2208804..2210150 + 1347 WP_061435137.1 precorrin-3B synthase -
AYR47_RS10210 2210143..2210769 + 627 WP_033897351.1 precorrin-8X methylmutase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-97)

Antitoxin

Uncharacterized protein HI1420

(2-94)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11729.17 Da        Isoelectric Point: 9.5427

>T61429 WP_061435131.1 NZ_CP014546:c2206486-2206178 [Pseudomonas azotoformans]
MIRFKRSDEFQEWLDSSRSRPARGRVLTRLDNATRGNFGDCAPVGNGVSEMRIHYGPGYRVYFTRVDEVVYLLLIGGDKS
TQKRDIQRAKEIADEFGIRNDS

Download         Length: 309 bp

>T61429 NZ_CP014546:c2206486-2206178 [Pseudomonas azotoformans]
ATGATTCGATTCAAGAGATCCGACGAGTTTCAAGAGTGGCTTGATTCGTCACGCAGCAGGCCAGCCCGCGGTCGTGTGTT
GACAAGATTGGATAACGCAACGAGGGGTAACTTCGGAGACTGTGCACCCGTAGGAAATGGCGTATCAGAAATGCGCATCC
ACTATGGCCCTGGATACCGGGTTTACTTCACACGCGTTGATGAGGTGGTTTACCTGCTGTTGATAGGTGGCGATAAATCC
ACGCAAAAACGCGACATCCAGCGCGCTAAAGAAATAGCCGATGAATTTGGAATAAGGAATGACTCATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10551.04 Da        Isoelectric Point: 5.1133

>AT61429 WP_061435130.1 NZ_CP014546:c2206181-2205891 [Pseudomonas azotoformans]
MTQFKDFDIAEHLKTPEDMAEYLEASFEEDSGDGLLIRSALNNIARAQGMTQIARDAGLGRESLYKALSATGNPEFATIM
KVMKALGLKFHATAIR

Download         Length: 291 bp

>AT61429 NZ_CP014546:c2206181-2205891 [Pseudomonas azotoformans]
ATGACTCAATTTAAAGACTTCGACATAGCTGAGCACCTCAAGACTCCCGAGGACATGGCCGAGTACCTTGAAGCCTCTTT
CGAAGAGGACTCCGGCGACGGCTTGCTGATTCGATCAGCCCTGAACAACATCGCCCGCGCCCAGGGCATGACCCAAATCG
CCCGCGACGCCGGCCTCGGCCGCGAAAGCCTGTACAAAGCCCTCTCCGCCACTGGTAATCCCGAATTCGCGACTATCATG
AAAGTCATGAAAGCGTTGGGCCTCAAGTTTCACGCCACCGCTATCCGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A127HVR9

Antitoxin

Source ID Structure
AlphaFold DB A0A127HWA5

References