Detailed information of TA system TA060720

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4945891..4946726
• Replicon: chromosome
• Accession: NZ_CP014316
• Organism: Escherichia coli JJ1887

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: AX202_RS24790
• Protein ID: WP_000854759.1
• Coordinates: 4946349..4946726 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: AX202_RS24785
• Protein ID: WP_001295723.1
• Coordinates: 4945891..4946259 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX202_RS24755	4943006..4943164	+	159	WP_001323397.1	DUF905 family protein	-
AX202_RS24760	4943319..4944137	+	819	WP_001234738.1	DUF945 domain-containing protein	-
AX202_RS24765	4944479..4944952	+	474	WP_001350782.1	antirestriction protein	-
AX202_RS24770	4944968..4945444	+	477	WP_001186775.1	RadC family protein	-
AX202_RS24775	4945507..4945728	+	222	WP_000692345.1	DUF987 domain-containing protein	-
AX202_RS24785	4945891..4946259	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AX202_RS24790	4946349..4946726	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
AX202_RS24795	4946723..4947211	+	489	WP_000761690.1	hypothetical protein	-
AX202_RS24800	4947228..4947404	+	177	WP_000839286.1	DUF957 domain-containing protein	-
AX202_RS30385	4947504..4947662	+	159	WP_001467148.1	hypothetical protein	-
AX202_RS30180	4948026..4948202	+	177	Protein_4720	helix-turn-helix domain-containing protein	-
AX202_RS24810	4948975..4950615	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4932197..4958372	26175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T60720 WP_000854759.1 NZ_CP014316:4946349-4946726 [Escherichia coli JJ1887]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T60720 NZ_CP014316:4946349-4946726 [Escherichia coli JJ1887]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT60720 WP_001295723.1 NZ_CP014316:4945891-4946259 [Escherichia coli JJ1887]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT60720 NZ_CP014316:4945891-4946259 [Escherichia coli JJ1887]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52