Detailed information of TA system TA060513

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 236654..237333
• Replicon: chromosome
• Accession: NZ_CP014270
• Organism: Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: DHB4_RS01140
• Protein ID: WP_000854672.1
• Coordinates: 236654..236995 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: DHB4_RS01145
• Protein ID: WP_000070395.1
• Coordinates: 237016..237333 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DHB4_RS01115	231931..232332	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
DHB4_RS01120	232371..233426	-	1056	WP_000749863.1	phosphoporin PhoE	-
DHB4_RS01125	233714..234817	+	1104	WP_001285288.1	glutamate 5-kinase	-
DHB4_RS01130	234829..236082	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
DHB4_RS01140	236654..236995	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
DHB4_RS01145	237016..237333	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
DHB4_RS23900	237352..237573	-	222	WP_000691994.1	DUF987 domain-containing protein	-
DHB4_RS01150	237582..238058	-	477	WP_000811693.1	RadC family protein	-
DHB4_RS01155	238074..238532	-	459	WP_000211838.1	antirestriction protein	-
DHB4_RS01160	238630..238869	-	240	WP_000194654.1	DUF905 domain-containing protein	-
DHB4_RS01165	238946..239413	-	468	WP_001547765.1	hypothetical protein	-
DHB4_RS26370	239436..239879	-	444	WP_000824223.1	hypothetical protein	-
DHB4_RS26375	239879..240106	-	228	WP_001548158.1	hypothetical protein	-
DHB4_RS26380	240102..240293	-	192	Protein_227	DeoR family transcriptional regulator	-
DHB4_RS01180	240510..241331	-	822	WP_000197389.1	DUF945 domain-containing protein	-
DHB4_RS01185	241423..242286	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T60513 WP_000854672.1 NZ_CP014270:c236995-236654 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T60513 NZ_CP014270:c236995-236654 [Escherichia coli K-12]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT60513 WP_000070395.1 NZ_CP014270:c237333-237016 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT60513 NZ_CP014270:c237333-237016 [Escherichia coli K-12]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684