Detailed information of TA system TA060320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 134673..135256
• Replicon: chromosome
• Accession: NZ_CP014163
• Organism: Aerococcus urinaehominis strain CCUG42038B

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A109RG51
• Locus tag: AWM75_RS00655
• Protein ID: WP_067977250.1
• Coordinates: 134909..135256 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AWM75_RS00650
• Protein ID: WP_074572463.1
• Coordinates: 134673..134909 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWM75_RS00635	129946..130596	-	651	WP_067977246.1	hypothetical protein	-
AWM75_RS00640	130876..132279	+	1404	WP_067977247.1	hypothetical protein	-
AWM75_RS00645	132320..134350	-	2031	WP_067977248.1	M13 family metallopeptidase	-
AWM75_RS00650	134673..134909	+	237	WP_074572463.1	hypothetical protein	Antitoxin
AWM75_RS00655	134909..135256	+	348	WP_067977250.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWM75_RS00660	135364..136464	+	1101	WP_067977251.1	glycerol dehydrogenase	-
AWM75_RS00665	136690..137886	+	1197	WP_067977252.1	cobalamin-independent methionine synthase II family protein	-
AWM75_RS00670	137965..138924	-	960	WP_074572442.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13172.49 Da
• Isoelectric Point: 10.5846

>T60320 WP_067977250.1 NZ_CP014163:134909-135256 [Aerococcus urinaehominis]
MAKTVRKYIPDKGDIVWIDFDPSAGHEINKRRPGLVVSRRNFNLATQFAVVCPITSTLKDLPTRYQLPGALTTKGQIVIS
QLKSLDFNHRRITFIEKLPANDLLVIDQMLKFIFF

Nucleotide

• Length: 348 bp

>T60320 NZ_CP014163:134909-135256 [Aerococcus urinaehominis]
ATGGCTAAGACAGTTAGAAAATACATACCGGATAAGGGTGATATTGTCTGGATTGATTTCGATCCTTCAGCTGGACATGA
GATTAACAAGAGAAGACCGGGACTGGTAGTATCCCGTCGTAATTTTAATCTAGCGACACAATTTGCCGTAGTCTGCCCAA
TTACCTCCACATTGAAGGACTTGCCGACCCGTTACCAGCTACCAGGTGCTTTAACCACTAAAGGGCAAATTGTGATATCT
CAACTCAAATCTTTAGACTTTAACCATCGACGCATCACTTTTATTGAAAAACTACCAGCCAATGATCTCTTAGTTATTGA
CCAAATGTTAAAATTTATATTTTTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8801.93 Da
• Isoelectric Point: 3.8206

>AT60320 WP_074572463.1 NZ_CP014163:134673-134909 [Aerococcus urinaehominis]
IMMLTTKTRKQGNSVVVTLPVDDEHVIQTQKEYIVTYTESGSIILTPIIADPFQVEEEGIYYEADVWADINQAGEELL

Nucleotide

• Length: 237 bp

>AT60320 NZ_CP014163:134673-134909 [Aerococcus urinaehominis]
ATAATGATGTTAACTACTAAAACACGTAAGCAAGGCAACTCTGTTGTCGTGACCCTGCCAGTGGATGATGAGCATGTGAT
ACAAACTCAAAAAGAGTATATCGTTACTTATACTGAGTCAGGATCAATTATTCTAACGCCAATTATTGCGGACCCTTTTC
AGGTTGAAGAAGAGGGCATCTATTACGAAGCAGATGTCTGGGCGGATATCAACCAAGCTGGCGAGGAATTACTTTAA

Structures

Toxin

• AlphaFold DB: A0A109RG51