Detailed information of TA system TA060258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Phd-LOTUS_5_Limkain_b1
• Location: 110657..111378
• Replicon: plasmid pTP143
• Accession: NZ_CP014142
• Organism: Thermus parvatiensis strain RL

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AV541_RS10765
• Protein ID: WP_014511248.1
• Coordinates: 110657..110965 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AV541_RS10770
• Protein ID: WP_014511247.1
• Coordinates: 110962..111378 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AV541_RS11325	106154..106255	-	102	Protein_108	LacI family DNA-binding transcriptional regulator	-
AV541_RS10740	106246..106785	+	540	Protein_109	IclR family transcriptional regulator	-
AV541_RS10745	107094..108261	+	1168	Protein_110	cytochrome P450	-
AV541_RS10750	108258..108956	+	699	WP_060384883.1	lycopene cyclase domain-containing protein	-
AV541_RS10755	108961..109634	+	674	Protein_112	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
AV541_RS10760	109631..110651	+	1021	Protein_113	glycosyltransferase	-
AV541_RS10765	110657..110965	+	309	WP_014511248.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Toxin
AV541_RS10770	110962..111378	+	417	WP_014511247.1	type II toxin-antitoxin system VapC family toxin	Antitoxin
AV541_RS10775	111476..113043	+	1568	Protein_116	phytoene desaturase	-
AV541_RS10780	113040..114035	+	996	Protein_117	type 2 isopentenyl-diphosphate Delta-isomerase	-
AV541_RS10785	114045..114515	+	471	WP_060384884.1	nucleotidyltransferase domain-containing protein	-
AV541_RS10790	114622..115746	+	1125	WP_008631498.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..143277	143277
	-	flank	IS/Tn	-	-	115683..117272	1589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (20-83)

Antitoxin

• PIN domain (3-129)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11641.34 Da
• Isoelectric Point: 5.2809

>T60258 WP_014511248.1 NZ_CP014142:110657-110965 [Thermus parvatiensis]
VASELDRPVFSVYPEGVKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKT
LPNEEVEALFQRQKTGFREVDF

Nucleotide

• Length: 309 bp

>T60258 NZ_CP014142:110657-110965 [Thermus parvatiensis]
GTGGCCTCTGAACTAGACAGACCGGTCTTTTCCGTGTACCCTGAGGGGGTGAAGGCCATCTGGCAACTCCAAGAGGCCAA
AGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCAGCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGG
TGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCTCGGTCCTCGAGGCCCTAAGACCCCCCAAGACC
CTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGCTTCCGGGAGGTGGACTTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15241.82 Da
• Isoelectric Point: 10.4393

>AT60258 WP_014511247.1 NZ_CP014142:110962-111378 [Thermus parvatiensis]
MRYLLDTNVVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAE
VMELWGELTGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 417 bp

>AT60258 NZ_CP014142:110962-111378 [Thermus parvatiensis]
CTGAGGTACCTGCTGGACACCAACGTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCG
CCACCTTAAAGTGGGGGAGGCGTACCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGG
CCCGAAGGGCCATCCTCAGCCGATGGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAG
GTGATGGAGCTCTGGGGCGAGCTCACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGC
GGCCACGGCCCTGCGGCACGGCCTGATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGA
ACCCCTGGGAAGGGTAA