Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 76700..77225 Replicon plasmid unnamed1
Accession NZ_CP014005
Organism Klebsiella pneumoniae subsp. pneumoniae strain NUHL24835

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag AWN66_RS26900 Protein ID WP_013023785.1
Coordinates 76920..77225 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AWN66_RS26895 Protein ID WP_001568025.1
Coordinates 76700..76918 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN66_RS26865 71856..72635 + 780 WP_013023780.1 hypothetical protein -
AWN66_RS26870 72842..74434 + 1593 WP_015344964.1 hypothetical protein -
AWN66_RS26875 74468..75595 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
AWN66_RS26880 75592..75882 + 291 WP_013023783.1 hypothetical protein -
AWN66_RS26895 76700..76918 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AWN66_RS26900 76920..77225 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
AWN66_RS26905 77394..77789 + 396 WP_017899885.1 hypothetical protein -
AWN66_RS28800 77816..78130 + 315 WP_053389906.1 hypothetical protein -
AWN66_RS26910 78141..79157 + 1017 WP_017899884.1 hypothetical protein -
AWN66_RS26915 79355..80149 + 795 WP_004197635.1 site-specific integrase -
AWN66_RS29085 80597..80776 - 180 WP_006788217.1 hypothetical protein -
AWN66_RS26920 80896..81522 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / aadA2 - 1..101030 101030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T59828 WP_013023785.1 NZ_CP014005:76920-77225 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T59828 NZ_CP014005:76920-77225 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT59828 WP_001568025.1 NZ_CP014005:76700-76918 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT59828 NZ_CP014005:76700-76918 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References