Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2343..2949 Replicon plasmid pPsCRG01-2
Accession NZ_CP013947
Organism Piscirickettsia salmonis strain PSCGR01

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B8UI87
Locus tag AWE47_RS17115 Protein ID WP_027242955.1
Coordinates 2343..2603 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7U4WZG4
Locus tag AWE47_RS17120 Protein ID WP_016211078.1
Coordinates 2596..2949 (+) Length 118 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWE47_RS19230 10..986 - 977 Protein_1 IS30 family transposase -
AWE47_RS17105 1099..1425 + 327 WP_196480238.1 hypothetical protein -
AWE47_RS17110 1525..2112 - 588 Protein_3 site-specific integrase -
AWE47_RS17115 2343..2603 + 261 WP_027242955.1 type II toxin-antitoxin system HicA family toxin Toxin
AWE47_RS17120 2596..2949 + 354 WP_016211078.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AWE47_RS19235 3126..4100 + 975 WP_081323805.1 IS30 family transposase -
AWE47_RS17130 4209..4436 + 228 Protein_7 hypothetical protein -
AWE47_RS17135 4633..4998 + 366 WP_027242954.1 hypothetical protein -
AWE47_RS17140 5143..5397 + 255 WP_027242953.1 type II toxin-antitoxin system HicA family toxin -
AWE47_RS17145 5390..5737 + 348 WP_027242952.1 type II toxin-antitoxin system HicB family antitoxin -
AWE47_RS19240 5835..6809 + 975 WP_036771330.1 IS30 family transposase -
AWE47_RS21215 7041..7187 - 147 WP_169834793.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33556 33556


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-82)

Antitoxin

Uncharacterised protein family HicB

(53-103)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9695.31 Da        Isoelectric Point: 10.9468

>T59640 WP_027242955.1 NZ_CP013947:2343-2603 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Download         Length: 261 bp

>T59640 NZ_CP013947:2343-2603 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 13492.39 Da        Isoelectric Point: 5.0200

>AT59640 WP_016211078.1 NZ_CP013947:2596-2949 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Download         Length: 354 bp

>AT59640 NZ_CP013947:2596-2949 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B8UI87

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4WZG4

References