Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 117127..117663 Replicon plasmid p4PS11
Accession NZ_CP013815
Organism Piscirickettsia salmonis strain AY3864B

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1L6THY5
Locus tag AVM73_RS17610 Protein ID WP_036772441.1
Coordinates 117391..117663 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag AVM73_RS17605 Protein ID WP_032126795.1
Coordinates 117127..117387 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVM73_RS17585 113478..114605 + 1128 WP_036772450.1 hypothetical protein -
AVM73_RS17590 114685..114948 + 264 Protein_147 IS6 family transposase -
AVM73_RS17595 115337..115768 + 432 Protein_148 IS6 family transposase -
AVM73_RS17600 115771..116337 - 567 Protein_149 IS6 family transposase -
AVM73_RS19190 116623..116787 - 165 Protein_150 IS6 family transposase -
AVM73_RS17605 117127..117387 + 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AVM73_RS17610 117391..117663 + 273 WP_036772441.1 Txe/YoeB family addiction module toxin Toxin
AVM73_RS17615 117739..118467 + 729 WP_017375910.1 IS6 family transposase -
AVM73_RS17620 119037..120101 - 1065 WP_174872554.1 IS91 family transposase -
AVM73_RS19870 120281..120460 - 180 WP_157894752.1 hypothetical protein -
AVM73_RS17635 120873..121700 + 828 WP_048876208.1 IS30 family transposase -
AVM73_RS17640 121743..122472 - 730 Protein_157 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..188300 188300
- inside IScluster/Tn - - 97498..128086 30588


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10785.31 Da        Isoelectric Point: 7.8926

>T59369 WP_036772441.1 NZ_CP013815:117391-117663 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Download         Length: 273 bp

>T59369 NZ_CP013815:117391-117663 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT59369 WP_032126795.1 NZ_CP013815:117127-117387 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT59369 NZ_CP013815:117127-117387 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6THY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References