Detailed information of TA system TA059365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 9911..10517
• Replicon: plasmid p4PS12
• Accession: NZ_CP013810
• Organism: Piscirickettsia salmonis strain PM31429B

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: AVM74_RS18095
• Protein ID: WP_027242955.1
• Coordinates: 10257..10517 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7U4WZG4
• Locus tag: AVM74_RS18090
• Protein ID: WP_016211078.1
• Coordinates: 9911..10264 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVM74_RS20535	5673..5819	+	147	WP_169834793.1	hypothetical protein	-
AVM74_RS18060	6051..7025	-	975	WP_036771330.1	IS30 family transposase	-
AVM74_RS18065	7123..7470	-	348	WP_027242952.1	type II toxin-antitoxin system HicB family antitoxin	-
AVM74_RS18070	7463..7717	-	255	WP_027242953.1	type II toxin-antitoxin system HicA family toxin	-
AVM74_RS18075	7862..8227	-	366	WP_027242954.1	hypothetical protein	-
AVM74_RS18080	8424..8651	-	228	Protein_14	hypothetical protein	-
AVM74_RS18085	8760..9734	-	975	WP_069971681.1	IS30 family transposase	-
AVM74_RS18090	9911..10264	-	354	WP_016211078.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AVM74_RS18095	10257..10517	-	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
AVM74_RS18100	10748..11338	+	591	WP_016212329.1	site-specific integrase	-
AVM74_RS18105	11404..11760	-	357	WP_075278739.1	transposase	-
AVM74_RS18110	11874..12848	+	975	WP_036771330.1	IS30 family transposase	-
AVM74_RS18115	12845..13288	-	444	WP_178099494.1	transposase	-
AVM74_RS20540	13493..13768	-	276	WP_171824216.1	hypothetical protein	-
AVM74_RS18125	14274..15257	-	984	WP_016211499.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33559	33559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T59365 WP_027242955.1 NZ_CP013810:c10517-10257 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T59365 NZ_CP013810:c10517-10257 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13492.39 Da
• Isoelectric Point: 5.0200

>AT59365 WP_016211078.1 NZ_CP013810:c10264-9911 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT59365 NZ_CP013810:c10264-9911 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87

Antitoxin

• AlphaFold DB: A0A7U4WZG4