Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 64469..65005 Replicon plasmid p1PS12
Accession NZ_CP013807
Organism Piscirickettsia salmonis strain PM31429B

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1L6THY5
Locus tag AVM74_RS16665 Protein ID WP_036772441.1
Coordinates 64469..64741 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag AVM74_RS16670 Protein ID WP_032126795.1
Coordinates 64745..65005 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVM74_RS16635 59660..60389 + 730 Protein_81 IS6 family transposase -
AVM74_RS16640 60432..61259 - 828 WP_048876208.1 IS30 family transposase -
AVM74_RS20490 61672..61851 + 180 WP_157894752.1 hypothetical protein -
AVM74_RS16655 62031..63095 + 1065 WP_174872554.1 IS91 family transposase -
AVM74_RS16660 63665..64393 - 729 WP_017375910.1 IS6 family transposase -
AVM74_RS16665 64469..64741 - 273 WP_036772441.1 Txe/YoeB family addiction module toxin Toxin
AVM74_RS16670 64745..65005 - 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AVM74_RS19780 65345..65509 + 165 Protein_88 IS6 family transposase -
AVM74_RS16680 65795..66361 + 567 Protein_89 IS6 family transposase -
AVM74_RS16685 66427..67059 - 633 Protein_90 IS6-like element ISPsa2 family transposase -
AVM74_RS19785 67129..67296 + 168 Protein_91 IS6 family transposase -
AVM74_RS16695 67347..67553 - 207 Protein_92 IS6 family transposase -
AVM74_RS16700 67637..68287 - 651 WP_155046631.1 hypothetical protein -
AVM74_RS16705 68362..69339 + 978 WP_036771347.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..181234 181234
- inside IScluster/Tn - - 54046..85394 31348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10785.31 Da        Isoelectric Point: 7.8926

>T59363 WP_036772441.1 NZ_CP013807:c64741-64469 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Download         Length: 273 bp

>T59363 NZ_CP013807:c64741-64469 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT59363 WP_032126795.1 NZ_CP013807:c65005-64745 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT59363 NZ_CP013807:c65005-64745 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6THY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References